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首页> 外文期刊>Journal of Molecular Biology >Accurate genetic switch in Escherichia coli: Novel mechanism of regulation by co-repressor
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Accurate genetic switch in Escherichia coli: Novel mechanism of regulation by co-repressor

机译：大肠杆菌中准确的基因转换：协同阻遏物调控的新机制

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摘要

Understanding a biological module involves recognition of its structure and the dynamics of its principal components. In this report we present an analysis of the dynamics of the repression module within the regulation of the trp operon in Escherichia coli. We combine biochemical data for reaction rate constants for the trp repressor binding to trp operator and in vivo data of a number of tryptophan repressors (TrpRs) that bind to the operator. The model of repression presented in this report greatly differs from previous mathematical models. One, two or three TrpRs can bind to the operator and repress the transcription. Moreover, reaction rates for detachment of TrpRs from the operator strongly depend on tryptophan (Trp) concentration, since Trp can also bind to the repressor-operator complex and stabilize it. From the mathematical modeling and analysis of reaction rates and equilibrium constants emerges a high-quality, accurate and effective module of trp repression. This genetic switch responds accurately to fast consumption of Trp from the interior of a cell. It switches with minimal dispersion when the concentration of Trp drops below a thousand molecules per cell. (C) 2008 Elsevier Ltd. All rights reserved.
机译：了解生物模块涉及对其结构及其主要成分的动力学的认识。在本报告中，我们对大肠杆菌中trp操纵子调节范围内阻抑模块的动力学进行了分析。我们结合生化数据的反应速率常数为trp阻遏物绑定到trp操作员和体内的许多色氨酸阻遏物（TrpRs）绑定到操作员的数据。本报告中介绍的压制模型与以前的数学模型有很大不同。一，两个或三个TrpR可以与操纵子结合并抑制转录。而且，由于Trp也可以结合阻遏物-操纵子复合物并使之稳定，因此从操纵子分离TrpR的反应速率在很大程度上取决于色氨酸（Trp）的浓度。通过对反应速率和平衡常数进行数学建模和分析，可以得出高质量，准确而有效的trp抑制模块。这种遗传转换对细胞内部Trp的快速消耗做出准确的反应。当Trp的浓度降至每个细胞一千个分子以下时，它将以最小的分散度进行切换。 （C）2008 Elsevier Ltd.保留所有权利。

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