• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of Molecular Biology >DNA combing reveals intrinsic temporal disorder in the replication of yeast chromosome VI
【24h】

DNA combing reveals intrinsic temporal disorder in the replication of yeast chromosome VI

机译：DNA梳理揭示了酵母第六号染色体复制中的内在时间紊乱

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

It is generally believed that DNA replication in most eukaryotes proceeds according to a precise program in which there is a defined temporal order by which each chromosomal region is duplicated. However, the regularity of this program at the level of individual chromosomes, in terms of both the relative timing and the size of the DNA domain, has not been addressed. Here, the replication of chromosome VI from synchronized budding yeast was studied at a resolution of similar to 1 kb with DNA combing and fluorescence microscopy. Contrary to what would be expected from cells following a rigorous temporal program, no two molecules exhibited the same replication pattern. Moreover, a direct evaluation of the extent to which the replication of distant chromosomal segments was coordinated indicates that the overwhelming majority of these segments were replicated independently. Importantly, averaging the patterns of all the fibers examined recapitulates the ensemble-averaged patterns obtained from population studies of the replication of chromosome VI. Thus, rather than an absolutely defined temporal order of replication, replication timing appears to be essentially probabilistic within individual cells, exhibiting only temporal tendencies within extended domains. (c) 2007 Elsevier Ltd. All rights reserved.
机译：通常认为，大多数真核生物中的DNA复制是根据精确的程序进行的，其中存在确定的时间顺序，每个染色体区域通过该时间顺序进行复制。但是，该程序在单个染色体水平上的规则性，无论是相对时间还是DNA结构域的大小，都没有得到解决。在这里，通过DNA梳理和荧光显微镜研究了同步芽芽酵母中染色体VI的复制，其分辨率接近1 kb。与遵循严格的时序程序的细胞所期望的相反，没有两个分子表现出相同的复制模式。此外，直接评估远距离染色体片段复制的协调程度表明，这些片段的绝大多数是独立复制的。重要的是，对所有检查到的纤维的图案进行平均，可以概括出从对VI染色体复制的总体研究中获得的整体平均图案。因此，复制时序似乎不是绝对限定的复制时间顺序，而是在单个细胞内基本上是概率性的，仅在扩展域内表现出时间趋势。 （c）2007 Elsevier Ltd.保留所有权利。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号