Confronting fusion protein-based membrane protein topology mapping with reality: the Escherichia coli ClcA H+/Cl- exchange transporter.

Cassel M; Seppala S; von-Heijne G

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Confronting fusion protein-based membrane protein topology mapping with reality: the Escherichia coli ClcA H+/Cl- exchange transporter.

机译：面对融合蛋白为基础的膜蛋白拓扑结构图与现实：大肠杆菌ClcA H + / Cl-交换转运蛋白。

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The topology of bacterial inner membrane proteins is commonly determined using topology reporters such as alkaline phosphatase and green fluorescent protein fused to a series of C-terminally truncated versions of the protein in question. Here, we report a detailed topology mapping of the Escherichia coli inner membrane H(+)/Cl(-) exchange transporter ClcA. Since the 3-D structure of ClcA is known, our results provide a critical test of the reporter fusion approach and offer new insights into the ClcA folding pathway.

机译：细菌内膜蛋白的拓扑结构通常是使用拓扑结构报告分子（例如碱性磷酸酶和绿色荧光蛋白）融合到相关蛋白的一系列C端截短形式来确定的。在这里，我们报告了大肠杆菌内膜H（+）/ Cl（-）交换转运蛋白ClcA的详细拓扑图。由于ClcA的3-D结构是已知的，我们的结果提供了对报告子融合方法的关键测试，并提供了有关ClcA折叠途径的新见解。

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《Journal of Molecular Biology》 |2008年第4期|共7页
作者
Cassel M; Seppala S; von-Heijne G;
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正文语种 eng
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关键词
Alkaline Phosphatase; Amino Acid Sequence; Antiporters; Escherichia coli; 碱性磷酸酶; 氨基酸序列; 反向转运物; 大肠杆菌; 基因; 报告; 离子泵; 膜蛋白质类;

机译：Alkaline Phosphatase;Amino Acid Sequence;Antiporters;Escherichia coli;碱性磷酸酶;氨基酸序列;反向转运物;大肠杆菌;基因;报告;离子泵;膜蛋白质类;

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1. Confronting fusion protein-based membrane protein topology mapping with reality: the Escherichia coli ClcA H+/Cl- exchange transporter. [J] . Cassel M, Seppala S, von-Heijne G Journal of Molecular Biology . 2008,第4期

机译：面对融合蛋白为基础的膜蛋白拓扑结构图与现实：大肠杆菌ClcA H + / Cl-交换转运蛋白。
2. Use of phoA fusions to study the topology of the Escherichia coli inner membrane protein leader peptidase. [J] . J L San Millan, D Boyd, R Dalbey, Journal of bacteriology . 1989,第10期

机译：phoA融合蛋白用于研究大肠杆菌内膜蛋白前导肽酶的拓扑结构。
3. Proteomic profile of osteoclast membrane proteins: identification of Na+/H+ exchanger domain containing 2 and its role in osteoclast fusion. [J] . Ha BG, Hong JM, Park JY, Proteomics . 2008,第13期

机译：破骨细胞膜蛋白的蛋白质组学概况：鉴定含有2的Na + / H +交换结构域及其在破骨细胞融合中的作用。
4. Full-length and truncated ice-nucleating protein-based cell-surface display of green fluorescent protein in Escherichia coli [C] . International Symposium on the Biosafety of Genetically Modified Organisms; 20021010-16; Beijing(CN) . 2002

机译：全长和截短的基于冰核蛋白的绿色荧光蛋白在大肠杆菌中的细胞表面展示
5. How did the substrate cross the membrane? Structural studies of the human erythrocyte anion exchanger and the Escherichia coli glycerol-3-phosphate transporter. [D] . Lemieux, Mary Joanne. 2003

机译：底物如何穿过膜？人类红细胞阴离子交换剂和大肠杆菌3-磷酸甘油转运蛋白的结构研究。
6. Rapid topology mapping of Escherichia coli inner-membrane proteins by prediction and PhoA/GFP fusion analysis [O] . David Drew, Dan Sjöstrand, Johan Nilsson, 2002

机译：通过预测和PhoA / GFP融合分析快速构建大肠杆菌内膜蛋白的拓扑图
7. Rapid topology mapping of Escherichia coli inner-membrane proteins by prediction and PhoA/GFP fusion analysis [O] . Drew, David, Sjöstrand, Dan, Nilsson, Johan, 2002

机译：通过预测和PhoA / GFP融合分析快速构建大肠杆菌内膜蛋白的拓扑图

Confronting fusion protein-based membrane protein topology mapping with reality: the Escherichia coli ClcA H+/Cl- exchange transporter.

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