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首页> 外文期刊>Journal of Molecular Biology >The integrity of the sarcin/ricin domain of 23 S ribosomal RNA is not required for elongation factor-in dependent peptide synthesis
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The integrity of the sarcin/ricin domain of 23 S ribosomal RNA is not required for elongation factor-in dependent peptide synthesis

机译：延伸因子依赖性肽合成不需要23 S核糖体RNA的sarcin / ricin结构域的完整性

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摘要

The elongation stage of protein synthesis consists of repeated cycles of the binding of aminoacyl-tRNA, peptide bond formation, and translocation. The process is normally catalyzed by the elongation factors Tu and G; however, the reactions can proceed, at least in prescribed and limited circumstance, in the absence of the elongation factors, a finding that strongly implies that the chemistry of protein synthesis is inherent in the ribosome. The sarcin/ricin domain in 23 S rRNA, the site of inactivation of ribosomes by ribotoxins, is where the elongation factors bind. The question that arises is whether the sarcin/ricin domain is necessary for factor-independent peptide synthesis. The answer is that it is not. The disruption of the sarcin/ ricin domain by covalent modification with either sarcin or pokeweed antiviral protein did not affect factor-independent peptide synthesis; nor did lethal mutations of nucleotides that abolish the binding of elongation factors. The results imply that the sole function of the sarcin/ricin domain is to provide a binding site for the elongation factors and, hence, to facilitate the elongation reactions. The results also raise the possibility of the co-evolution of the sarcin/ricin domain and the elongation factors.
机译：蛋白质合成的延长阶段包括氨酰基-tRNA结合，肽键形成和易位的重复循环。该过程通常由伸长因子Tu和G催化;然而，在没有延伸因子的情况下，反应至少可以在规定和有限的情况下进行，这一发现强烈暗示着蛋白质合成的化学是核糖体固有的。 23 S rRNA中的sarcin / ricin结构域是核糖毒素使核糖体失活的位点，是延伸因子结合的地方。出现的问题是，sarcin / ricin结构域是否是非因子依赖性肽合成所必需的。答案是事实并非如此。通过用sarcin或商陆抗病毒蛋白进行共价修饰来破坏sarcin / ricin结构域，不会影响因子独立的肽合成。消除延伸因子结合的核苷酸的致命突变也没有。结果暗示，sarcin /蓖麻毒蛋白结构域的唯一功能是提供延伸因子的结合位点，从而促进延伸反应。结果还增加了sarcin / ricin结构域和延伸因子共同进化的可能性。

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