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首页> 外文期刊>Journal of Molecular Biology >Heterocyst-specific transcription of NsiR1, a non-coding RNA encoded in a tandem array of direct repeats in cyanobacteria.
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Heterocyst-specific transcription of NsiR1, a non-coding RNA encoded in a tandem array of direct repeats in cyanobacteria.

机译：NsiR1的异胚性转录，NsiR1是在蓝细菌中直接重复串联排列的非编码RNA。

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In response to nitrogen deficiency, some cyanobacteria develop heterocysts, a terminally differentiated cell type, specialized for the fixation of atmospheric nitrogen. In Nostocales, this differentiation process is controlled by two major regulators, NtcA and HetR, but additional unknown factors are likely to be involved as well. In the context of a genome-wide search for potential non-coding RNAs, we identified an array of 12 tandem repeats that is transcribed in large amounts when cells enter conditions that trigger cell differentiation and switch to nitrogen fixation. The main accumulating transcript, which we suggest designating nitrogen stress-induced RNA 1 (NsiR1), has properties similar to regulatory non-coding RNAs. In Anabaena sp. PCC 7120, it is about 60 nt in length, has a very distinct predicted secondary structure, and is expressed very early and transiently after nitrogen step-down. Moreover, its expression requires HetR and NtcA and is restricted to cells that are differentiating into heterocysts, clearly placing NsiR1 within the regulon that controls the switch to nitrogen fixation and heterocyst formation. The genomic arrangement of NsiR1, located upstream of hetF, a gene whose product is involved in heterocyst formation, is conserved in all five Nostocales whose genomes are completely sequenced. Additionally, we detected NsiR1 expression in 19 different heterocyst-forming cyanobacteria. Our data suggest that every repeat is a complete transcriptional unit furnished with a cell-type-specific promoter and a Rho-independent terminator, which gives rise to a very high NsiR1 transcript level. NsiR1 is the first known bacterial non-coding RNA that is specifically upregulated in response to nitrogen step-down.
机译：响应氮缺乏，一些蓝细菌会形成异种囊，这是一种终末分化的细胞类型，专门用于固定大气中的氮。在Nostocales中，这种分化过程受两个主要调控因子NtcA和HetR的控制，但是其他未知因素也可能会参与其中。在全基因组范围内寻找潜在的非编码RNA的背景下，我们确定了12个串联重复序列的阵列，当细胞进入触发细胞分化并转换为固氮的条件时，该序列会大量转录。我们建议指定氮胁迫诱导的RNA 1（NsiR1）为主要积累转录本，其性质类似于非编码调控性RNA。在鱼腥藻中PCC 7120的长度约为60 nt，具有非常不同的预测二级结构，并且在降氮后非常早且短暂地表达。此外，其表达需要HetR和NtcA，并且仅限于分化为异胞藻的细胞，从而将NsiR1清楚地置于调节到固氮和异胞藻形成转换的调节子中。 NsiR1的基因组排列位于hetF的上游，hetF是一个其产物参与异囊形成的基因，在所有五个基因组均已完全测序的Nostocales中是保守的。此外，我们检测到NsiR1在19种不同的形成异囊藻的蓝细菌中的表达。我们的数据表明，每个重复序列都是一个完整的转录单位，配有细胞类型特异性启动子和不依赖Rho的终止子，从而产生了很高的NsiR1转录水平。 NsiR1是第一个已知的细菌非编码RNA，响应于氮的递减特别上调。

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