• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of Molecular Biology >A mechanism of release of calreticulin from cells during apoptosis.
【24h】

A mechanism of release of calreticulin from cells during apoptosis.

机译：凋亡过程中钙网蛋白从细胞释放的机制。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Calreticulin (CRT) is an endoplasmic reticulum (ER) chaperone responsible for glycoprotein folding and Ca(2+) homeostasis. CRT also has extracellular functions, e.g. tumor and apoptotic cell recognition and wound healing, but the mechanism of CRT extracellular release is unknown. Cytosolic localization of CRT is determined by signal peptide and subsequent retrotranslocation of CRT into the cytoplasm. Here, we show that under apoptotic stress conditions, the cytosolic concentration of CRT increases and associates with phosphatidylserine (PS) in a Ca(2)(+)-dependent manner. PS distribution is regulated by aminophospholipid translocase (APLT), which maintains PS on the cytosolic side of the cell membrane. APLT is sensitive to redox modifications of its SH groups by reactive nitrogen species. During apoptosis, both CRT expression and the concentration of nitric oxide (NO) increase. By using S-nitroso-l-cysteine-ethyl-ester, an intracellular NO donor and inhibitor of APLT, we showed that PS and CRT externalization occurred together in an S-nitrosothiol-dependent and caspase-independent manner. Furthermore, the CRT and PS are relocated as punctate clusters on the cell surface. Thus, CRT induced nitrosylation and its externalization with PS could explain how CRT acts as a bridging molecule during apoptotic cell clearance.
机译：钙网蛋白（CRT）是内质网（ER）伴侣，负责糖蛋白折叠和Ca（2+）稳态。 CRT还具有细胞外功能，例如肿瘤和凋亡细胞识别和伤口愈合，但CRT细胞外释放的机制尚不清楚。 CRT的胞质定位由信号肽和随后的CRT逆向转运到细胞质中来确定。在这里，我们表明，在细胞凋亡的应激条件下，CRT的胞质浓度增加并与Ca（2）（+）依赖性方式的磷脂酰丝氨酸（PS）关联。 PS的分布受氨基磷脂转位酶（APLT）的调节，后者使PS保持在细胞膜的胞质侧。 APLT对反应性氮物种对其SH基团的氧化还原修饰很敏感。在细胞凋亡期间，CRT表达和一氧化氮（NO）浓度均增加。通过使用S-亚硝基-1-半胱氨酸-乙酯，一种细胞内NO供体和APLT抑制剂，我们显示PS和CRT外在化以S-亚硝基硫醇依赖性和caspase依赖性方式一起发生。此外，CRT和PS作为点状簇重新定位在单元表面上。因此，CRT诱导的亚硝基化作用及其在PS中的外在作用可以解释CRT在凋亡细胞清除过程中如何作为桥接分子。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号