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首页> 外文期刊>Journal of Molecular Biology >Structural studies of ROK fructokinase YdhR from Bacillus subtilis: insights into substrate binding and fructose specificity.
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Structural studies of ROK fructokinase YdhR from Bacillus subtilis: insights into substrate binding and fructose specificity.

机译：枯草芽孢杆菌ROK果糖激酶YdhR的结构研究：底物结合和果糖特异性的见解。

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摘要

The main pathway of bacterial sugar phosphorylation utilizes specific phosphoenolpyruvate phosphotransferase system (PTS) enzymes. In addition to the classic PTS system, a PTS-independent secondary system has been described in which nucleotide-dependent sugar kinases are used for monosaccharide phosphorylation. Fructokinase (FK), which phosphorylates d-fructose with ATP as a cofactor, has been shown to be a member of this secondary system. Bioinformatic analysis has shown that FK is a member of the "ROK" (bacterial Repressors, uncharacterized Open reading frames, and sugar Kinases) sequence family. In this study, we report the crystal structures of ROK FK from Bacillus subtilis (YdhR) (a) apo and in the presence of (b) ADP and (c) ADP/d-fructose. All structures show that YdhR is a homodimer with a monomer composed of two similar alpha/beta domains forming a large cleft between domains that bind ADP and D-fructose. Enzymatic activity assays support YdhR function as an ATP-dependent fructose kinase.
机译：细菌糖磷酸化的主要途径利用特定的磷酸烯醇丙酮酸磷酸转移酶系统（PTS）酶。除了经典的PTS系统外，还描述了一种不依赖PTS的二级系统，其中核苷酸依赖性糖激酶用于单糖磷酸化。果糖激酶（FK）以ATP为辅因子磷酸化果糖，已被证明是该次要系统的成员。生物信息学分析表明FK是“ ROK”（细菌阻遏物，未鉴定的开放阅读框和糖激酶）序列家族的成员。在这项研究中，我们报告了来自枯草芽孢杆菌（YdhR）（a）apo和（b）ADP和（c）ADP / d-果糖存在下ROK FK的晶体结构。所有结构均显示YdhR是同型二聚体，其单体由两个相似的α/β结构域组成，在结合ADP和D-果糖的结构域之间形成大裂缝。酶活性测定支持YdhR作为ATP依赖性果糖激酶的功能。

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