• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of Molecular Biology >Drosophila BTB/POZ domains of 'ttk group' can form multimers and selectively interact with each other.
【24h】

Drosophila BTB/POZ domains of 'ttk group' can form multimers and selectively interact with each other.

机译：“ ttk基”的果蝇BTB / POZ结构域可形成多聚体并选择性地彼此相互作用。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The BTB (bric-a-brac, tramtrack and broad complex)/POZ (poxvirus and zinc finger) domain is a conserved protein-protein interaction motif contained in a variety of transcription factors involved in development, chromatin remodeling, insulator activity, and carcinogenesis. All well-studied mammalian BTB domains form obligate homodimers and, rarely, tetramers. Only the BTB domain of the Drosophila GAGA factor (GAF) has been shown to exist as higher-order multimers. The BTB domain of GAF belongs to the "ttk group" that contains several highly conserved sequences not found in other BTB domains. Here, we have shown by size-exclusion chromatography, chemical cross-linking, and nondenaturing PAGE that four additional BTB domains of the ttk group-Batman, Mod(mdg4), Pipsqueak, and Tramtrack-can form multimers, like GAF. Interestingly, the BTB domains of GAF and Batman have formed a wide range of complexes and interacted in the yeast two-hybrid assay with other BTB domains tested. In contrast, the BTB domains of Mod(mdg4), Pipsqueak, and Tramtrack have formed stable high-order multimer complexes and failed to interact with each other. The BTB domain of Drosophila CP190 protein does not belong to the ttk group. This BTB domain has formed stable dimers and has not interacted with domains of the ttk group. Previously, it was suggested that GAF oligomerization into higher-order complexes facilitates long-range activation by providing a protein bridge between an enhancer and a promoter. Unexpectedly, experiments in the Drosophila model system have not supported the role of GAF in organization of long-distance interaction between the yeast GAL4 activator and the white promoter.
机译：BTB（金砖四国，tramtrack和广泛的复合物）/ POZ（痘病毒和锌指）结构域是一种保守的蛋白质-蛋白质相互作用基序，包含在涉及发育，染色质重塑，绝缘子活性和致癌作用的多种转录因子中。所有经过充分研究的哺乳动物BTB结构域均形成专性同二聚体，很少形成四聚体。仅果蝇GAGA因子（GAF）的BTB结构域已显示为高阶多聚体。 GAF的BTB域属于“ ttk组”，其中包含一些在其他BTB域中找不到的高度保守的序列。在这里，我们通过大小排阻色谱，化学交联和非变性PAGE显示，ttk组的四个其他BTB域-蝙蝠侠，Mod（mdg4），Pipsqueak和Tramtrack-可以形成多聚体，如GAF。有趣的是，GAF和Batman的BTB结构域已经形成了广泛的复合物，并且在酵母双杂交检测中与其他测试的BTB结构域发生了相互作用。相反，Mod（mdg4），Pipsqueak和Tramtrack的BTB域已形成稳定的高阶多聚体复合物，并且彼此之间无法相互作用。果蝇CP190蛋白的BTB结构域不属于ttk组。该BTB结构域已形成稳定的二聚体，并且未与ttk基团的结构域相互作用。以前，有人建议将GAF寡聚为高级复合物，可通过在增强子和启动子之间提供蛋白质桥来促进远程激活。出乎意料的是，果蝇模型系统中的实验并未支持GAF在酵母GAL4激活剂和白色启动子之间长距离相互作用的组织中的作用。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号