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首页> 外文期刊>Journal of Molecular Biology >Three-dimensional analysis of the association of viral particles with mitochondria during the replication of Rice gall dwarf virus.
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Three-dimensional analysis of the association of viral particles with mitochondria during the replication of Rice gall dwarf virus.

机译：稻gall矮病毒复制过程中病毒颗粒与线粒体结合的三维分析。

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摘要

Examination of cultured insect vector cells that had been infected with Rice gall dwarf virus (RGDV), using transmission electron microscopy and confocal microscopy, revealed the presence of clusters of virus-coated mitochondria around viroplasms in which replication and assembly of RGDV occurred, suggesting a role for mitochondria in supplying the energy required for viral morphogenetic processes. Electron tomography revealed that RGDV particles on the surface of mitochondria are arrayed in an orderly but loose manner, unlike tightly packaged particles in vesicular compartments, suggesting the presence of counterpart molecules on the surface of mitochondria. The viral particles in close proximity to mitochondria were aligned along intermediate filaments, which might serve as scaffolds for the anchorage of these particles. RGDV has a putative mitochondrion-targeting sequence on the outer surface of the outer-capsid protein P8. The arrangement of RGDV particles around mitochondria suggests that the region of the P8 protein containing the mitochondrion-targeting sequence might attach to a molecule like a receptor on the outer mitochondrial membrane. Our analysis demonstrates the three-dimensional arrangement and molecular basis for the mitochondrial proximity of RGDV particles during viral replication.
机译：使用透射电子显微镜和共聚焦显微镜检查了已感染稻胆矮病毒（RGDV）的培养昆虫载体细胞，发现病毒质膜周围线粒体簇存在，其中RGDV发生了复制和装配，提示线粒体在提供病毒形态发生过程所需能量方面的作用。电子断层扫描显示，线粒体表面上的RGDV颗粒以有序但疏松的方式排列，这与囊泡隔室中紧密包装的颗粒不同，表明线粒体表面上存在对应分子。紧密靠近线粒体的病毒颗粒沿着中间细丝排列，中间细丝可以充当这些颗粒锚定的支架。 RGDV在衣壳蛋白P8的外表面具有假定的线粒体靶向序列。线粒体周围RGDV颗粒的排列表明，包含线粒体靶向序列的P8蛋白区域可能附着在线粒体外膜上的类似受体的分子上。我们的分析证明了在病毒复制过程中RGDV颗粒线粒体接近的三维排列和分子基础。

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