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首页> 外文期刊>Journal of Molecular Biology >YajL, the prokaryotic homolog of the Parkinsonism-associated protein DJ-1, protects cells against protein sulfenylation
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YajL, the prokaryotic homolog of the Parkinsonism-associated protein DJ-1, protects cells against protein sulfenylation

机译：YajL是与帕金森氏症相关的蛋白质DJ-1的原核同源物，可保护细胞免于蛋白质亚磺酰化

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摘要

YajL is the closest Escherichia coli homolog of the Parkinsonism-associated protein DJ-1, a multifunctional oxidative stress response protein whose biochemical function remains unclear. We recently described the oxidative-stress-dependent aggregation of proteins in yajL mutants and the oxidative-stress-dependent formation of mixed disulfides between YajL and members of the thiol proteome. We report here that yajL mutants display increased protein sulfenic acids levels and that formation of mixed disulfides between YajL and its protein substrates in vivo is inhibited by the sulfenic acid reactant dimedone, suggesting that YajL preferentially forms disulfides with sulfenylated proteins. YajL (but not YajL C106A) also forms mixed disulfides in vitro with the sulfenylated form of bovine serum albumin. The YajL-serum albumin disulfides can be subsequently reduced by glutathione or dihydrolipoic acid. We also show that DJ-1 can form mixed disulfides with sulfenylated E. coli proteins and with sulfenylated serum albumin. These results suggest that YajL and possibly DJ-1 function as covalent chaperones involved in the detection of sulfenylated proteins by forming mixed disulfides with them and that these disulfides are subsequently reduced by low-molecular-weight thiols.
机译：YajL是帕金森氏症相关蛋白DJ-1（一种多功能的氧化应激反应蛋白，其生化功能尚不清楚）中与大肠杆菌最接近的同源物。我们最近描述了yajL突变体中蛋白质的氧化应激依赖性聚集以及YajL与巯基蛋白质组成员之间混合二硫化物的氧化应激依赖性形成。我们在这里报告yajL突变体显示增加的蛋白质亚硫酸水平，并且YajL与它的蛋白质底物在体内的体内混合二硫化物的形成受到亚磺酸反应物二甲酮的抑制，表明YajL优先与亚磺酰化的蛋白质形成二硫化物。 YajL（但不是YajL C106A）在体外也与牛血清白蛋白的亚磺酰化形式形成混合的二硫化物。随后可以通过谷胱甘肽或二氢硫辛酸还原YajL-血清白蛋白二硫化物。我们还显示DJ-1可以与亚磺化大肠杆菌蛋白和亚磺酰血清白蛋白混合形成二硫化物。这些结果表明，YajL和可能的DJ-1通过与它们形成混合的二硫化物而充当了检测亚磺化蛋白质的共价伴侣，并且随后这些二硫化物被低分子量的硫醇还原了。

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