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首页> 外文期刊>Journal of Molecular Biology >The structure of Xis reveals the basis for filament formation and insight into DNA bending within a mycobacteriophage intasome
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The structure of Xis reveals the basis for filament formation and insight into DNA bending within a mycobacteriophage intasome

机译：Xis的结构揭示了细丝形成的基础，并洞察了分枝杆菌噬菌体内体中的DNA弯曲

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摘要

The recombination directionality factor, Xis, is a DNA bending protein that determines the outcome of integrase-mediated site-specific recombination by redesign of higher-order protein-DNA architectures. Although the attachment site DNA of mycobacteriophage Pukovnik is likely to contain four sites for Xis binding, Xis crystals contain five subunits in the asymmetric unit, four of which align into a Xis filament and a fifth that is generated by an unusual domain swap. Extensive intersubunit contacts stabilize a bent filament-like arrangement with Xis monomers aligned head to tail. The structure implies a DNA bend of ~ 120, which is in agreement with DNA bending measured in vitro. Formation of attR-containing intasomes requires only Int and Xis, distinguishing Pukovnik from lambda. Therefore, we conclude that, in Pukovnik, Xis-induced DNA bending is sufficient to promote intramolecular Int-mediated bridges during intasome formation.
机译：重组方向因子Xis是一种DNA弯曲蛋白，通过重新设计高阶蛋白质DNA结构来确定整合酶介导的位点特异性重组的结果。尽管分枝杆菌噬菌体普科夫尼克的附着位点DNA可能包含四个Xis结合位点，但Xis晶体在不对称单元中包含五个亚基，其中四个排列成一个Xis细丝，而另一个则由异常的结构域交换产生。广泛的亚基间接触可稳定弯曲的丝状排列，使Xis单体头尾对齐。该结构暗示DNA弯曲约为120，与体外测得的DNA弯曲一致。包含attR的内含体的形成仅需要Int和Xis，即可将Pukovnik与lambda区分开。因此，我们得出的结论是，在Pukovnik中，Xis诱导的DNA弯曲足以在Intasome形成过程中促进分子内Int介导的桥。

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