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首页> 外文期刊>Journal of Molecular Biology >Influence of Internal DNA Pressure on Stability and Infectivity of Phage lambda
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Influence of Internal DNA Pressure on Stability and Infectivity of Phage lambda

机译：内部DNA压力对λ噬菌体稳定性和感染性的影响

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摘要

Viruses must remain infectious while in harsh extracellular environments. An important aspect of viral particle stability for double-stranded DNA viruses is the energetically unfavorable state of the tightly confined DNA chain within the virus capsid creating pressures of tens of atmospheres. Here, we study the influence of internal genome pressure on the thermal stability of viral particles. Using differential scanning calorimetry to monitor genome loss upon heating, we find that internal pressure destabilizes the virion, resulting in a smaller activation energy barrier to trigger DNA release. These experiments are complemented by plaque assay and electron microscopy measurements to determine the influence of intra-capsid DNA pressure on the rates of viral infectivity loss. At higher temperatures (65-75 degrees C), failure to retain the packaged genome is the dominant mechanism of viral inactivation. Conversely, at lower temperatures (40-55 degrees C), a separate inactivation mechanism dominates, which results in non-infectious particles that still retain their packaged DNA. Most significantly, both mechanisms of infectivity loss are directly influenced by internal DNA pressure, with higher pressure resulting in a more rapid rate of inactivation at all temperatures. (c) 2015 Elsevier Ltd. All rights reserved.
机译：在恶劣的细胞外环境中，病毒必须保持传染性。对于双链DNA病毒，病毒颗粒稳定性的一个重要方面是病毒衣壳内紧密限制的DNA链在能量上不利的状态，从而产生数十个大气压。在这里，我们研究内部基因组压力对病毒颗粒热稳定性的影响。使用差示扫描量热法监测加热时的基因组丢失，我们发现内部压力使病毒体不稳定，从而导致较小的激活能垒来触发DNA释放。这些实验辅以噬斑测定和电子显微镜测量来确定衣壳内DNA压力对病毒感染性丧失速率的影响。在较高温度（65-75摄氏度）下，无法保留包装的基因组是病毒灭活的主要机制。相反，在较低的温度（40-55摄氏度）下，一个独立的灭活机制起着主导作用，这导致非感染性颗粒仍然保留了其包装的DNA。最重要的是，两种感染力丧失的机制都直接受到内部DNA压力的影响，而更高的压力导致在所有温度下的灭活速度都更快。 （c）2015 Elsevier Ltd.保留所有权利。

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