Quantitative analysis of SecYEG-mediated insertion of transmembrane α-helices into the bacterial inner membrane

?jemalmK.; BotelhoS.C.; StüdleC.; VonHeijneG.

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Quantitative analysis of SecYEG-mediated insertion of transmembrane α-helices into the bacterial inner membrane

机译：SecYEG介导的跨膜α螺旋插入细菌内膜的定量分析

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Most integral membrane proteins, both in prokaryotic and eukaryotic cells, are co-translationally inserted into the membrane via Sec-type translocons: the SecYEG complex in prokaryotes and the Sec61 complex in eukaryotes. The contributions of individual amino acids to the overall free energy of membrane insertion of single transmembrane α-helices have been measured for Sec61-mediated insertion into the endoplasmic reticulum (ER) membrane (Nature 450:1026-1030) but have not been systematically determined for SecYEG-mediated insertion into the bacterial inner membrane. We now report such measurements, carried out in Escherichia coli. Overall, there is a good correlation between the results found for the mammalian ER and the E. coli inner membrane, but the hydrophobicity threshold for SecYEG-mediated insertion is distinctly lower than that for Sec61-mediated insertion.

机译：在原核和真核细胞中，大多数完整的膜蛋白都通过Sec型转录子共翻译插入膜中：原核生物中的SecYEG复合体和真核生物中的Sec61复合体。对于Sec61介导的插入内质网（ER）膜，已经测量了单个氨基酸对单个跨膜α螺旋膜插入总自由能的贡献（自然450：1026-1030），但尚未系统确定用于SecYEG介导的插入细菌内膜。我们现在报告在大肠杆菌中进行的此类测量。总体而言，在哺乳动物内质网和大肠杆菌内膜之间发现了良好的相关性，但是SecYEG介导的插入的疏水性阈值明显低于Sec61介导的插入的疏水性阈值。

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《Journal of Molecular Biology》 |2013年第15期|共10页
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?jemalmK.; BotelhoS.C.; StüdleC.; VonHeijneG.;
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peptidase membrane protein; SecYEG leader; transmembrane helix;

机译：肽酶膜蛋白;SecYEG前导分子;跨膜螺旋;

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1. Quantitative analysis of SecYEG-mediated insertion of transmembrane α-helices into the bacterial inner membrane [J] . ?jemalmK., BotelhoS.C., StüdleC., Journal of Molecular Biology . 2013,第15期

机译：SecYEG介导的跨膜α螺旋插入细菌内膜的定量分析
2. Effects of Flanking Loops on Membrane Insertion of Transmembrane Helices: A Role for Peptide Conformational Equilibrium [J] . Jian Gao, Jianhan Chen The journal of physical chemistry, B. Condensed matter, materials, surfaces, interfaces & biophysical . 2013,第28期

机译：侧翼环对跨膜螺旋膜插入的影响：肽构象平衡的作用。
3. Effects of Flanking Loops on Membrane Insertion of Transmembrane Helices: A Role for Peptide Conformational Equilibrium [J] . Jian Gao, Jianhan Chen The journal of physical chemistry, B. Condensed matter, materials, surfaces, interfaces & biophysical . 2013,第28期

机译：侧翼环对跨膜螺旋膜插入的影响：肽构象平衡的作用。
4. Evaluation of lateral interactions between membrane gangliosidesand receptor transmembrane segments in lipid bilayers based onsemi-quantitative fluorescence analysis [C] . Mikito Nakano, Shinya Hanashima, Toshiaki Hara 日本化学会春季年会 . 2020

机译：基于血液定量荧光分析的脂质双层膜神经植物和受体跨膜段横向相互作用的评价
5. Functional consequences of charged amino acid substitutions in and around transmembrane helices in the third membrane spanning domain of MRP1. [D] . Situ, Donna. 2004

机译：MRP1第三跨膜结构域中跨膜螺旋内部和周围带电氨基酸取代的功能后果。
6. ER membrane protein complex is required for the insertions of late-synthesized transmembrane helices of Rh1 in Drosophila photoreceptors [O] . Naoki Hiramatsu, Tatsuya Tago, Takunori Satoh, 2019

机译：ER膜蛋白复合物是插入后期合成Rh1跨膜螺旋所需的果蝇感光器
7. Quantitative Analysis of SecYEG-Mediated Insertion of Transmembrane α-Helices into the Bacterial Inner Membrane [O] . Öjemalm Karin, Botelho Salomé Calado, Stüdle Chiara, 2013

机译：SecYEG介导跨膜α螺旋菌插入细菌内膜的定量分析。

Quantitative analysis of SecYEG-mediated insertion of transmembrane α-helices into the bacterial inner membrane

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