• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of Molecular Biology >Identification of a GUAAY Pentaloop Sequence Involved in a Novel RNA Loop-Helix Interaction
【24h】

Identification of a GUAAY Pentaloop Sequence Involved in a Novel RNA Loop-Helix Interaction

机译：涉及新型RNA Loop-螺旋相互作用的GUAAY五环序列的鉴定。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Large RNAs often utilize GNRA tetraloops as structural elements to stabilize the overall tertiary fold. These tetraloop-receptor (TR) interactions have a conserved geometry in which the tetraloop docks into the receptor at an angle of similar to 15 degrees from the helix containing the receptor. Here, we show that the conserved GUAAY pentaloop found in domain III of group IIB1 introns participates in a novel class of RNA tertiary interaction with a geometry and mode of binding that are significantly different from that found in GNRA TR interactions. This pentaloop is highly conserved within the IIB1 class and interacts with the minor groove of the catalytic domain V. The base planes of the loop and receptor nucleotides are not coplanar and greatly deviate from standard A-minor motifs. The helical axis of the GUAAY stem loop diverges similar to 70 degrees from the angle of insertion found in a typical GNRA TR interaction. Therefore, the loop architecture and insertion orientation are distinctive, with in vitro splicing data indicating that a GNRA tetraloop is incompatible at this position. The GUAAY pentaloop-receptor motif is also found in the structure of the eukaryotic thiamine pyrophosphate riboswitch in the context of a hexanucleotide loop sequence. We therefore propose, based on phylogenetic, structural, and biochemical data, that the GUAAY pentaloop-receptor interaction represents a novel structural motif that is present in multiple structured RNAs. (C) 2016 Elsevier Ltd. All rights reserved.
机译：大型RNA通常利用GNRA四环作为结构元件来稳定总体三级折叠。这些四环-受体（TR）相互作用具有保守的几何结构，其中四环以与包含受体的螺旋线相似的15度角进入受体。在这里，我们显示在IIB1组内含子的结构域III中发现的保守GUAAY pentaloop参与了一类新颖的RNA三级相互作用，其几何结构和结合模式与GNRA TR相互作用中发现的显着不同。这种五倍体在IIB1类中是高度保守的，并且与催化结构域V的小沟相互作用。环和受体核苷酸的底面不是共面的，并且与标准的A-次要基序大不相同。 GUAAY茎环的螺旋轴与典型GNRA TR相互作用中发现的插入角相差约70度。因此，环的结构和插入方向是独特的，体外剪接数据表明GNRA四环在该位置不相容。在六核苷酸环序列的背景下，在真核硫胺硫胺焦磷酸盐核糖开关的结构中也发现了GUAAY五肽受体基序。因此，我们建议，基于系统发育，结构和生化数据，GUAAY pentaloop-receptor相互作用代表了存在于多个结构化RNA中的新型结构基序。 （C）2016 Elsevier Ltd.保留所有权利。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号