• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of Neurochemistry: Offical Journal of the International Society for Neurochemistry >Cholesterol regulates multiple forms of vesicle endocytosis at a mammalian central synapse
【24h】

Cholesterol regulates multiple forms of vesicle endocytosis at a mammalian central synapse

机译：胆固醇调节哺乳动物中央突触中多种形式的囊泡内吞作用

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Endocytosis in synapses sustains neurotransmission by recycling vesicle membrane and maintaining the homeostasis of synaptic membrane. A role of membrane cholesterol in synaptic endocytosis remains controversial because of conflicting observations, technical limitations in previous studies, and potential interference from non-specific effects after cholesterol manipulation. Furthermore, it remains unclear whether cholesterol participates in distinct forms of endocytosis that function under different activity levels. In this study, applying the whole-cell membrane capacitance measurement to monitor endocytosis in real time at the rat calyx of Held terminals, we found that disrupting cholesterol with dialysis of cholesterol oxidase or methyl--cyclodextrin impaired three different forms of endocytosis, including slow endocytosis, rapid endocytosis, and endocytosis of the retrievable membrane that exists at the surface before stimulation. The effects were observed when disruption of cholesterol was mild enough not to change Ca2+ channel current or vesicle exocytosis, indicative of stringent cholesterol requirement in synaptic endocytosis. Extracting cholesterol with high concentrations of methyl--cyclodextrin reduced exocytosis, mainly by decreasing the readily releasable pool and the vesicle replenishment after readily releasable pool depletion. Our study suggests that cholesterol is an important, universal regulator in multiple forms of vesicle endocytosis at mammalian central synapses.
机译：突触中的内吞作用通过回收囊泡膜并维持突触膜的稳态来维持神经传递。膜胆固醇在突触内吞中的作用仍存在争议，因为观察结果矛盾，先前研究中的技术局限性以及胆固醇操作后非特异性作用的潜在干扰。此外，尚不清楚胆固醇是否参与在不同活性水平下起作用的不同形式的内吞作用。在这项研究中，应用全细胞膜电容测量实时监测Held末端大鼠花萼中的内吞作用，我们发现通过透析胆固醇氧化酶或甲基环糊精破坏胆固醇会破坏三种不同形式的内吞作用，包括缓慢内吞，快速内吞和刺激前存在于表面的可回收膜的内吞。当胆固醇的破坏足够轻微而不会改变Ca2 +通道电流或囊泡胞吐作用时，会观察到这种影响，这表明突触内吞作用中胆固醇的严格要求。用高浓度的甲基-环糊精提取胆固醇可减少胞吐作用，主要是通过减少易于释放的库和在易于释放的库消耗后减少囊泡的补充。我们的研究表明，胆固醇是哺乳动物中央突触中多种形式的囊泡内吞作用的重要通用调节剂。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号