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首页> 外文期刊>Journal of Neurophysiology >Organization of pontine reticulospinal inputs to motoneurons controlling axial and limb muscles in the neonatal mouse
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Organization of pontine reticulospinal inputs to motoneurons controlling axial and limb muscles in the neonatal mouse

机译:脑桥网状脊髓输入到运动神经元控制新生小鼠的轴向和四肢肌肉

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摘要

Using optical recording of synaptically mediated calcium transients and selective spinal lesions, we investigated the pattern of activation of spinal motoneurons (MNs) by the pontine reticulospinal projection in isolated brain stem-spinal cord preparations from the neonatal mouse. Stimulation sites throughout the region where the pontine reticulospinal neurons reside reliably activated MNs at cervical, thoracic, and lumbar levels. Activation was similar in MNs ipsi- and contralateral to the stimulation site, similar in medial and lateral motor columns that contain trunk and limb MNs, respectively, and similar in the L2 and L5 segments that predominantly contain flexor and extensor MNs, respectively. In nonlesioned preparations, responses in both ipsi- and contralateral MNs followed individual stimuli in stimulus trains nearly one-to-one (with few failures). After unilateral hemisection at C1 on the same side as the stimulation, responses had substantially smaller magnitudes and longer latencies and no longer followed individual stimuli. After unilateral hemisection at C1 on the side opposite to the stimulation, the responses were also smaller, but their latencies were not affected. Thus we distinguish two pontine reticulospinal pathways to spinal MNs, one uncrossed and the other crossed, of which the uncrossed pathway transmits more faithfully and appears to be more direct.
机译:使用光学记录的突触介导的钙瞬变和选择性的脊柱病变,我们调查了桥脑网状脊髓投射在新生小鼠分离的脑干-脊髓制备中的脊髓运动神经元(MNs)的激活模式。在脑桥,胸椎和腰椎水平,桥脑网状脊髓神经元所处的整个区域的刺激部位均可靠地激活了MN。在与刺激部位同侧和对侧的MN中,激活相似,分别在包含躯干和四肢MN的内侧和外侧运动柱中相似,在主要包含屈肌和伸张MN的L2和L5节中相似。在非损伤性制剂中,同种和对侧MN中的反应都在刺激序列中的单个刺激之后几乎一对一地发生(几乎没有失败)。在与刺激相同的一侧在C1处进行单侧半切后,反应的幅度明显较小,潜伏期更长,并且不再遵循单个刺激。在与刺激相反的一侧在C1进行单侧半切后,反应也较小,但潜伏期不受影响。因此,我们区分出两个桥脑网状脊髓通路到脊柱MN,一个未交叉且另一个交叉,其中未交叉的通路更加忠实地传递并且似乎更直接。

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