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首页> 外文期刊>Journal of Neurophysiology >Neuron-glia cross talk revealed in reverberating networks by simultaneous extracellular recording of spikes and astrocytes' glutamate transporter and K+ currents
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Neuron-glia cross talk revealed in reverberating networks by simultaneous extracellular recording of spikes and astrocytes' glutamate transporter and K+ currents

机译：通过在细胞外同时记录尖峰和星形胶质细胞的谷氨酸转运蛋白和K +电流，在回响网络中揭示了神经元胶质细胞的串扰

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Astrocytes uptake synaptically released glutamate with electrogenic transporters (GluT) and buffer the spike-dependent extracellular K+ excess with background K+ channels. We studied neuronal spikes and the slower astrocytic signals on reverberating neocortical cultures and organotypic slices from mouse brains. Spike trains and glial responses were simultaneously captured from individual sites of multielectrode arrays (MEA) by splitting the recorded traces into appropriate filters and reconstructing the original signal by deconvolution. GluT currents were identified by using DL-threo-beta-benzyloxyaspartate (TBOA). K+ currents were blocked by 30 mu M Ba2+, suggesting a major contribution of inwardly rectifying K+ currents. Both types of current were tightly correlated with the spike rate, and their astrocytic origin was tested in primary cultures by blocking glial proliferation with cytosine beta-D-arabinofuranoside (AraC). The spike-related, time-locked inward and outward K+ currents in different regions of the astrocyte syncytium were consistent with the assumptions of the spatial K+ buffering model. In organotypic slices from ventral tegmental area and prefrontal cortex, the GluT current amplitudes exceeded those observed in primary cultures by several orders of magnitude, which allowed to directly measure transporter currents with a single electrode. Simultaneously measuring cell signals displaying widely different amplitudes and kinetics will help clarify the neuron-glia interplay and make it possible to follow the cross talk between different cell types in excitable as well as nonexcitable tissue.
机译：星形胶质细胞通过电转运蛋白（GluT）摄取谷氨酸盐，并利用背景K +通道缓冲过量的穗依赖性细胞外K +。我们研究了神经元的尖峰和较慢的星形细胞信号在回荡小鼠大脑的新皮层文化和器官型切片上的作用。通过将记录的迹线分成适当的滤波器并通过反卷积重建原始信号，可以同时从多电极阵列（MEA）的各个位置捕获尖峰序列和神经胶质反应。通过使用DL-苏-β-苄氧基天冬氨酸（TBOA）识别GluT电流。 K +电流被30μM Ba2 +阻断，表明向内整流K +电流的主要贡献。两种电流都与尖峰频率紧密相关，并且通过使用胞嘧啶β-D-阿拉伯呋喃糖苷（AraC）阻断神经胶质细胞的增殖，在原代培养中测试了它们的星形细胞起源。星形胶质合胞体不同区域中与尖峰相关的，时间锁定的向内和向外的钾离子电流与空间钾离子缓冲模型的假设一致。在来自腹侧被盖区和前额叶皮层的器官型切片中，GluT电流幅度比原代培养中观察到的幅度高出几个数量级，这使得可以用单个电极直接测量转运蛋白电流。同时测量显示出很大幅度和动力学变化的细胞信号将有助于弄清神经胶质细胞之间的相互作用，并使得有可能追踪兴奋性组织和非兴奋性组织中不同细胞类型之间的串扰。

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