Involvement of membrane-type bile acid receptor M-BAR/TGR5 in bile acid-induced activation of epidermal growth factor receptor and mitogen-activated protein kinases in gastric carcinoma cells.

Yasuda H; Hirata S; Inoue K; Mashima H; Ohnishi H; Yoshiba M

首页> 外文期刊>Biochemical and Biophysical Research Communications >Involvement of membrane-type bile acid receptor M-BAR/TGR5 in bile acid-induced activation of epidermal growth factor receptor and mitogen-activated protein kinases in gastric carcinoma cells.

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Involvement of membrane-type bile acid receptor M-BAR/TGR5 in bile acid-induced activation of epidermal growth factor receptor and mitogen-activated protein kinases in gastric carcinoma cells.

机译：膜型胆汁酸受体M-BAR / TGR5参与胆汁酸诱导的胃癌细胞中表皮生长因子受体和丝裂原活化蛋白激酶的活化。

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Bile acids, which have been implicated in gastrointestinal-tract cell carcinogenesis, share properties with tumor promoters in that both affect signal transduction pathways responsible for cell proliferation and apoptosis. In the present study, we demonstrate that EGFR-ERK1/2 is activated following treatment of AGS human gastric carcinoma cells with bile acids. EGFR phosphoactivation is ligand-dependent, since treatment of cells with HB-EGF antisera or CM197 (a selective inhibitor of HB-EGF) markedly inhibits deoxycholate (DC)-promoted activation. Membrane-type bile acid receptor (M-BAR)/TGR5 is a recently identified G-protein-coupled receptor (GPCR). In AGS cells, siRNAs that target M-BAR suppress DC-induced phosphorylation of EGFR. Furthermore, introduction of siRNAs targeting ADAM17 transcripts resulted in suppression of DC-induced activation of EGFR and ERK1/2. These results suggest that in AGS cells, DC transactivates EGFR through M-BAR- and ADAM/HB-EGF-dependent mechanisms.

机译：胆汁酸与胃肠道细胞癌变有关，它与肿瘤启动子具有共同的特性，因为它们都影响负责细胞增殖和凋亡的信号转导途径。在本研究中，我们证明在用胆汁酸治疗AGS人胃癌细胞后，EGFR-ERK1 / 2被激活。 EGFR磷酸化激活依赖于配体，因为用HB-EGF抗血清或CM197（HB-EGF的选择性抑制剂）处理细胞可显着抑制脱氧胆酸盐（DC）促进的激活。膜型胆汁酸受体（M-BAR）/ TGR5是最近发现的G蛋白偶联受体（GPCR）。在AGS细胞中，靶向M-BAR的siRNA抑制DC诱导的EGFR磷酸化。此外，针对ADAM17转录本的siRNA的引入导致抑制DC诱导的EGFR和ERK1 / 2激活。这些结果表明，在AGS细胞中，DC通过M-BAR和ADAM / HB-EGF依赖性机制使EGFR活化。

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《Biochemical and Biophysical Research Communications》 |2007年第1期|共6页
作者
Yasuda H; Hirata S; Inoue K; Mashima H; Ohnishi H; Yoshiba M;
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正文语种 eng
中图分类生物化学;
关键词
Bile Acids; Bar form; c-erbB-1 Proto-Oncogenes; receptor;

机译：胆汁酸;棒状;c-erbB-1原癌基因;受体;

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1. Involvement of membrane-type bile acid receptor M-BAR/TGR5 in bile acid-induced activation of epidermal growth factor receptor and mitogen-activated protein kinases in gastric carcinoma cells. [J] . Yasuda H, Hirata S, Inoue K, Biochemical and Biophysical Research Communications . 2007,第1期

机译：膜型胆汁酸受体M-BAR / TGR5参与胆汁酸诱导的胃癌细胞中表皮生长因子受体和丝裂原活化蛋白激酶的活化。
2. Parallel contractile signal transduction pathways activated by receptors for thrombin and epidermal growth factor-urogastrone in guinea pig gastric smooth muscle: blockade by inhibitors of mitogen-activated protein kinase-kinase and phosphatidyl inos [J] . Zheng XL, Renaux B, Hollenberg MD The Journal of Pharmacology and Experimental Therapeutics: Official Publication of the American Society for Pharmacology and Experimental Therapeutics . 1998,第1期

机译：凝血酶和表皮生长因子-尿激素在豚鼠胃平滑肌中激活的平行收缩信号转导途径：丝裂原活化蛋白激酶激酶和磷脂酰肌醇抑制剂的阻断
3. The platelet-derived-growth-factor receptor, not the epidermal-growth-factor receptor, is used by lysophosphatidic acid to activate p42/44 mitogen-activated protein kinase and to induce prostaglandin G/H synthase-2 in mesangial cells [J] . Christian O. A. REISER, Margarete GOPPELT-STRUEBE, Stefanie FICKEL The biochemical journal . 2000,第2期

机译：溶血磷脂酸使用血小板衍生的生长因子受体而非表皮生长因子受体来激活肾小球膜细胞中的p42 / 44丝裂原活化蛋白激酶并诱导前列腺素G / H合酶-2
4. Role of mitogen-activated protein kinases in tauroursodeoxycholic acid-induced bile secretion in cholestatic rat liver [C] . S. HOHENESTER, G. U. DENK, R.WIMMER, Falk Symposium . 2005

机译：促丝膜激活蛋白激酶在胆固醇大鼠肝脏诱导肺泡酸诱导的胆汁分泌中的作用
5. Polyunsaturated fatty acids and the epidermal growth factor receptor/mitogen-activated protein kinase signal transduction cascade in mammary cancer. [D] . Phipps, Brandy Ellen. 2004

机译：多不饱和脂肪酸和表皮生长因子受体/丝裂原活化蛋白激酶信号转导在乳腺癌中的级联作用。
6. The platelet-derived-growth-factor receptor not the epidermal-growth-factor receptor is used by lysophosphatidic acid to activate p42/44 mitogen-activated protein kinase and to induce prostaglandin G/H synthase-2 in mesangial cells. [O] . M Goppelt-Struebe, S Fickel, C O Reiser 2000

机译：溶血磷脂酸使用血小板衍生的生长因子受体而非表皮生长因子受体来激活p42 / 44丝裂原激活的蛋白激酶并诱导肾小球膜细胞中的前列腺素G / H合酶-2。
7. The platelet-derived-growth-factor receptor, not the epidermal-growth-factor receptor, is used by lysophosphatidic acid to activate p42/44 mitogen-activated protein kinase and to induce prostaglandin G/H synthase-2 in mesangial cells. [O] . Goppelt-Struebe, M, Fickel, S, Reiser, C O 2000

机译：溶血磷脂酸使用血小板衍生的生长因子受体而非表皮生长因子受体来激活p42 / 44丝裂原激活的蛋白激酶并诱导肾小球膜细胞中的前列腺素G / H合酶-2。

Involvement of membrane-type bile acid receptor M-BAR/TGR5 in bile acid-induced activation of epidermal growth factor receptor and mitogen-activated protein kinases in gastric carcinoma cells.

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