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首页> 外文期刊>International Journal of Biological Macromolecules: Structure, Function and Interactions >Voltammetric detection of miRNA hybridization based on electroactive indicator-cobalt phenanthroline
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Voltammetric detection of miRNA hybridization based on electroactive indicator-cobalt phenanthroline

机译：基于电活性指示剂 - 钴素的miRNA杂交伏安检测

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摘要

The indicator-based nucleic acid detection protocol is one of the major approaches to monitor the sequence selective nucleic acid hybridization-mediated recognition events in biochemical analysis. The metal complex, cobalt phenanthroline, [Co(phen)(3)(3+)], which is one of the electroactive indicators, interacts more with double stranded nucleic acids via intercalation. Thus, this interaction permits an increase at the electrochemical signal of [Co(phen)(3)(3+)]. In our study, the interaction of metal complex, [Co(phen)(3)(3+)] with nucleic acids was examined using pencil graphite electrodes (PGEs) in combination with differential pulse voltammetry (DPV) technique. The voltammetric detection of miRNA-34a was investigated based on the changes at the electrochemical signal of [Co(phen)(3)(3+)] under optimized experimental conditions; such as accumulation potentialof metal complex and DNA probe concentration, hybridization time, target miRNA concentration. Furthermore, the selectivity of electrochemical miRNA-34a biosensor was studied in contrast to different miRNAs. The applicability of indicator-based biosensor specific to miRNA-34a was also presented by using total RNA samples. (C) 2020 Elsevier B.V. All rights reserved.
机译：基于指示剂的核酸检测方案是监测生化分析中序列选择性核酸杂交介导的识别事件的主要方法之一。金属络合物，钴素，[Co（Phen）（3）（3）（3）]，其是电活性指示剂之一，通过插层与双链核酸相互作用。因此，该相互作用允许在CO（Phen）（3）（3）（3））的电化学信号的增加。在我们的研究中，使用铅笔石墨电极（PGES）与差分脉冲伏安法（DPV）技术相结合地检查金属络合物的相互作用[CO（Phen）（3）（3）（3）（3）]]。根据优化的实验条件，基于[CO（Phen）（3）（3）（3）]电化学信号的变化来研究MiRNA-34A的伏安检测;如累积电位，金属配合物和DNA探针浓度，杂交时间，靶miRNA浓度。此外，与不同的miRNA相比，研究了电化学miRNA-34A-生物传感器的选择性。还通过使用总RNA样品呈现了基于指示剂的生物传感器的适用性。 （c）2020 Elsevier B.v.保留所有权利。

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