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首页> 外文期刊>International Journal of Biological Macromolecules: Structure, Function and Interactions >An amperometric biosensor for specific detection of glycated hemoglobin based on recombinant engineered fructosyl peptide oxidase
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An amperometric biosensor for specific detection of glycated hemoglobin based on recombinant engineered fructosyl peptide oxidase

机译：基于重组工程化果糖肽氧化酶的糖化血红蛋白的特异性检测血管传感器

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摘要

Here, we present a specific biosensor based on the detection of glycated hemoglobin (HbA1c) proteolytic digestion product, fructosyl valyl histidine (Fru-ValHis). A recombinant engineered fructosyl peptide oxi-dase (FPDX) enzyme with improved specificity was immobilized on the electrode surface modified by chitosan (CHIT), graphene oxide (GO) and gold nanoparticles (AuNPs). The biosensor exhibited a linear response toward different concentrations of Fru-ValHis ranging from 0.1 to 2 mM with a sensitivity of 8.45 mu A mM(-1) cm(-2). Detection limit of the current biosensor for Fru-ValHis was 0.3 mu M as the lowest quantity required giving a signal to a background. Analytical recovery of added Fru-ValHis in whole blood was 95.1-98.35% for FPDX/AuNPs/GO/CHIT/FTO electrode. For Fru-ValHis determination by FPOX-AuNPs-GO-CHIT/FTO electrode, within-run coefficient of variation (CV) was between 1.3% and 2.4% and between run CV was between 2.1% and 3.5%. A significant change in electron transfer resistance after the incuba-tion of FPDX-modified electrode with Fru-ValHis was observed, while no response was achieved with control, indicating specific measurement of Fru-ValHis. Moreover, designed biosensor measured HbAlc in human blood samples and the results were well agreed with that obtained with NORUDIA (TM) HbA1c diagnostic kit. Overall, suitable specificity of the engineered FPDX made the bioelectrode responded well to the Fru-ValHis level, which demonstrates a promising application for specific detection of HbA1c biomarker. (C) 2019 Elsevier B.V. All rights reserved.
机译：这里，我们基于糖化血红蛋白（HBA1C）蛋白水解消化产物，果糖壬基组氨酸（FRU-VALHIS）的检测来介绍特异性生物传感器。将具有改善的特异性的重组工程果糖基肽氧化氧基 - Dase（FPDX）酶固定在用壳聚糖（Chit），石墨烯氧化物（GO）和金纳米粒子（AUNP）改性的电极表面上。生物传感器对不同浓度的FRU-VALHIS的线性反应，含量为0.1至2mm，灵敏度为8.45μmmm（-1）cm（-2）。 FRU-VALHIS的当前生物传感器的检测极限为0.3μm，因为所需的最低量为背景提供信号。对于FPDX / AUNPS / GO / CHIT / FTO电极，全血中添加的FRU-VALHIS的分析恢复为95.1-98.35％。对于FPOX-AUNPS-GO-CHIT / FTO电极的FRU-VALHIS测定，在运行内变化系数（CV）在1.3％和2.4％之间，运行CV之间的介于2.1％和3.5％之间。观察到FPDX改性电极与FRU-VALHIS孵化后的电子传递抗性的显着变化，同时对照进行对照，表明FRU-VALHIS的特异性测量。此外，设计的生物传感器在人血样中测量HBALC，结果与Norudia（TM）HBA1C诊断试剂盒获得的结果很好。总体而言，工程化FPDX的合适特异性使得生物电极对FRU-VALHIS水平响应，这证明了对HBA1C生物标志物的特异性检测的有望应用。 （c）2019 Elsevier B.v.保留所有权利。

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