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首页> 外文期刊>International Journal of Biological Macromolecules: Structure, Function and Interactions >Determination of bioethanol production potential from lignocellulosic biomass using novel Cel-5m isolated from cow rumen metagenome
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Determination of bioethanol production potential from lignocellulosic biomass using novel Cel-5m isolated from cow rumen metagenome

机译：用新型CEL-5M与牛瘤胃组成的新锡 - 5m测定木质纤维素生物量的生物乙醇生产潜力

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摘要

We are reporting the novel cellulase named Cel-5M from rumen metagenome. The deduced amino acid sequence and biochemical characterization suggested that Cel-5M is endoglucanase from the GH5 family with multifunctional potential Cel-5M showed similarity to non-characterized proteins and the genus Prevotella as parental organism. The 957 bp ORF encoding Cel-5M was cloned and overexpressed in E. coli and purified. The recombinant Cel-5M showed maximum activity at pH 6.0 and 40 degrees C. It retained more than 80% activity between 4 and 7 pH and 65% thermostability between 30 and 70 degrees C. Cel-5M showed activity on various substrates like CMC, Filter paper, Avicel, Xylan, beta-Mannan, and Glucopyranoside, which confirmed its multifunctional characteristics and classifies as member of subfamily 4 of GH5 (GH5_4). LCB hydrolysis potential of Cel-5M was studied using wheat straw (10% w/v). Alkali-treated and steam-exploded wheat straws were inoculated with 1 mg/g Cel-5M, 2% yeast in a reaction mixture and SSF at 10% wilt loading rate. The ethanol yield 0.46 g/g and 0.43 g/g of cellulose obtained after 72 h fermentation in alkali-treated and steam-exploded wheat straw, respectively. Cel-5M is novel multifunction cellulase belongs GH-5 endoglucanase from rumen origin can be employed for bioethanol based biofuel production. (C) 2019 Elsevier B.V. All rights reserved.
机译：我们报道了来自瘤胃梅塔豆蔻瘤中的Cel-5m的新型纤维素酶。推导的氨基酸序列和生物化学表征表明，Cel-5M是来自GH5家族的内切葡聚糖，其中多功能潜在的CEL-5M与非特征蛋白和PREVOTELLA属的相似性，作为父母生物体。在大肠杆菌中克隆并过表达了957bp ORF编码锡 - 5m并纯化。重组CEL-5M在pH6.0和40℃下显示最大活性。它在30至7 pH的4至7 pH值之间保持超过80％的活性，在30至70摄氏度之间的65％的热稳定性之间。CEL-5M在像CMC这样的各种衬底上显示出活性，滤纸，玻璃纤维，木聚糖，β-甘露苷和葡糖吡喃糖苷，证实了其多功能特征，并将其分类为GH5（GH5_4）的亚家族4的成员。使用小麦秸秆（10％w / v）研究Cel-5m的LCB水解电位。在反应混合物中用1mg / g cel-5m，2％酵母接种碱处理和蒸汽爆炸的小麦秸秆，并以10％的枯萎负载率的SSF接种。乙醇产率为0.46g / g和0.43g / g在72小时发酵碱处理和蒸汽爆炸麦秸后获得的纤维素。 CEL-5M是新型多功能纤维素酶属于瘤胃突发的GH-5内葡聚糖酶可用于基于生物乙醇的生物燃料生产。 （c）2019 Elsevier B.v.保留所有权利。

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