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首页> 外文期刊>International Journal of Biological Macromolecules: Structure, Function and Interactions >Double crosslinked HLC-CCS hydrogel tissue engineering scaffold for skin wound healing
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Double crosslinked HLC-CCS hydrogel tissue engineering scaffold for skin wound healing

机译：双交联HLC-CCS水凝胶组织工程支架用于皮肤伤口愈合

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摘要

Skin defects caused by various reasons are currently common clinical problems. At present, hydrogels have been proposed as tissue-engineered skin scaffolds to regenerate the tissues of the defect. We used human-like collagen (HLC), which was isolated and purified after high-density fermentation of recombinant E. coli BL21 The gel uses HLC and carboxymethylated chitosan (CCS) as raw materials and combines enzyme-chemical double cross-linking technology to form a three-dimensional porous network structure that mimics the human extracellular matrix, providing attachment points and nutrients for cell growth and proliferation. For comparison, we used a common hydrogel raw material, gelatin, to prepare a hydrogel in the same way. The experimental results show that the HLC-CCS skin scaffold hydrogel has good mechanical properties, high porosity and good histocompatibility. And full-thickness skin defect repair experiments showthat this hydrogel has a good ability to promote skin tissue regeneration at the wound. In summary, this HLC-based double-crosslinked hydrogel can be used as a project strategy for skin defect repair. (C) 2020 Elsevier B.V. All rights reserved.
机译：由于各种原因引起的皮肤缺陷是目前常见的临床问题。目前，已提出水凝胶作为组织工程皮肤支架，以再生缺陷的组织。我们使用人样胶原蛋白（HLC），其在重组大肠杆菌BL21的高密度发酵后被隔离和纯化，凝胶使用HLC和羧甲基化壳聚糖（CCS）作为原料，并将酶 - 化学双交联技术结合在一起形成一种三维多孔网络结构，用于模拟人体细胞外基质，为细胞生长和增殖提供附着点和营养素。为了比较，我们使用了常见的水凝胶原料，明胶，以相同的方式制备水凝胶。实验结果表明，HLC-CCS皮肤支架水凝胶具有良好的机械性能，高孔隙率和良好的组织相容性。和全厚的皮肤缺陷修复实验表明，该水凝胶具有良好的促进伤口皮肤组织再生能力。总之，这种基于HLC的双交联水凝胶可以用作皮肤缺陷修复的项目策略。 （c）2020 Elsevier B.v.保留所有权利。

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