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首页> 外文期刊>International Journal of Biological Macromolecules: Structure, Function and Interactions >Pullulanase activity: A novel indicator of inherent resistant starch in rice (Oryza sativa. L)
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Pullulanase activity: A novel indicator of inherent resistant starch in rice (Oryza sativa. L)

机译：淀粉酶活性：水稻中固有抗性淀粉的新型指标（Oryza Sativa。L）

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摘要

Starch quality studies over the decades highlighted the immense health benefits of resistant starch (RS), but still much is needed to elucidate the science behind its inherent formation. Till date, there is no report to establish the relationship between inherent RS content and pullulanase (PUL) activity in any of the crops. In this study, we emphasize the novel role of debranching enzyme, PUL towards inherent RS formation, using rice as a model crop. Biochemical analysis of 51 rice genotypes for amylose content (AC) revealed a good amount of variation ranging from 7.03 to 37.33%. Further, hierarchical clustering which resulted in 11 genotypes of varying RS (0.33-2.7%), highlighted medium dependency towards amylose and low dependency towards amylopectin content. The discrete differences in microstructure, unimodal distribution and tight packing of starch granules observed in higher RS genotype indicated the higher possibility of compact cluster structure of amylopectin, modulated by PUL. Qualitative and quantitative assays performed validated the relevant role of PUL towards inherent RS content with very high dependency score (R-2 = 0.98). This is the first report regarding the fact that higher PUL activity contribute to inherent RS using novel hypothetical 'Pullulanase-Amylopectin Trimming Model'. (C) 2019 Elsevier B.V. All rights reserved.
机译：过去的几十年淀粉品质的研究强调了抗性淀粉（RS）的巨大健康益处，但仍然有许多需要澄清其背后的内在形成的科学。截止日期，也没有报告建立在任何农作物的固有RS含量和支链淀粉酶（PUL）活动之间的关系。在这项研究中，我们强调脱支酶的新作用，对PUL固有RS的形成，使用米作为模式植物。的51个基因型直链淀粉含量（AC）的生化分析显示变化的范围从7.03到37.33％的良好的量。此外，这导致变化的RS（0.33-2.7％）的11种基因型分层聚类，突出向直链淀粉和低依赖性朝向支链淀粉含量介质依赖性。在显微组织，单峰分布和更高的RS观察到的淀粉颗粒的紧密堆积的离散差表示基因分支链淀粉的紧凑簇结构，通过PUL调制的较高可能性。定性和定量分析来进行验证PUL朝向固有RS内容的相关作用具有非常高的相关性得分（R-2 = 0.98）。这是关于一个事实，即较高的PUL活动使用新颖的假想的支链淀粉酶，支链淀粉微调模式“有助于固有RS的第一份报告。 （c）2019 Elsevier B.v.保留所有权利。

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