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首页> 外文期刊>International Journal of Biological Macromolecules: Structure, Function and Interactions >Regioselective sulfation of beta-glucan from Ganoderma lucidum and structure-anticoagulant activity relationship of sulfated derivatives
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Regioselective sulfation of beta-glucan from Ganoderma lucidum and structure-anticoagulant activity relationship of sulfated derivatives

机译：来自灵芝的β-葡聚糖的区域选择性硫酸和硫酸衍生物的结构 - 抗凝活性关系

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摘要

In the present study, regioselective sulfation of beta-glucan (GLP) from Ganoderma lucidum were firstly established by using 4,4'-dimethoxytrityl chloride and hexamethyldisilazane as protecting precursor. 2,4,6-O-sulfated, 6-O-sulfated and 2,4-O-sulfated GLP derivatives were prepared and the molecular weights (M-w) of derivatives were determined to range from 0.94 x 10(4) to 6.27 x 10(4) g/mol, while the degrees of sulfation (DS) were calculated to vary from 0.83 to 1.74. The regioselective sulfation of GLP was confirmed by FT-IR, C-13 NMR spectroscopy and methylation analysis. Results indicated that the sulfated substitution sites were predominantly at C-6 in 6-O-sulfated GLP (S(6-O)GLP) and C-4 in 2,4-O-sulfated GLP (S(2,4-O)GLP), respectively. Clotting assays (APTT, PT and TT) in vitro showed that sulfate groups were essential for anticoagulant activity and S(6-O)GLP exhibited much higher than others. Meanwhile, sulfated GLP with higher DS and M-w showed stronger anticoagulant activity in the case of the same condition. (C) 2020 Elsevier B.V. All rights reserved.
机译：在本研究中，首先通过使用4,4'-二甲氧基三氯酰氯和六甲基二硅氮烷作为保护前体，首先建立来自灵芝的β-葡聚糖（GLP）的区域硫化。制备2,4,6- O-硫酸化，6- o-硫酸化和2,4- o-硫酸化GLP衍生物，测定衍生物的分子量（MW）为0.94×10（4）至6.27 x 10（4）克/摩尔，而计算硫化程度（DS）以0.83至1.74的变化。通过FT-IR，C-13 NMR光谱和甲基化分析证实了GLP的区域选​​择性硫酸化。结果表明，硫酸化取代位点主要在6-O-硫酸化GLP中的C-6（S（6-O）GLP）和2,4-O-硫酸化GLP中的C-4（2,4-O. PLP）分别。体外凝血测定（APTT，PT和TT）显示硫酸盐基团对抗凝血活性至关重要，并且S（6 o）GLP比其它更高。同时，具有较高DS和M-W的硫酸化GLP在相同条件下显示出更强的抗凝血活性。 （c）2020 Elsevier B.v.保留所有权利。

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