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首页> 外文期刊>International Journal of Biological Macromolecules: Structure, Function and Interactions >Functional characterization of the promoter of pearl millet heat shock protein 10 (PgHsp10) in response to abiotic stresses in transgenic tobacco plants
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Functional characterization of the promoter of pearl millet heat shock protein 10 (PgHsp10) in response to abiotic stresses in transgenic tobacco plants

机译：珍珠米热休克蛋白10（PGHSP10）响应转基因烟草植物中非生物应力的功能表征

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摘要

In the present study, the promoter region of the pearl millet heat shock protein 10 (PgHsp10) gene was doned and characterized. The PgHsp10 promoter (PgHsp10pro) sequence region has all the cis-motifs required for tissue and abiotic stress inducibility. The complete PgHsp10pro (PgHsp10PC) region and a series of 5' truncations of PgHsp10 (PgHsp10D1 and PgHsp10D2) and an antisense form of PgHsp10pro (PgHsp10AS) were cloned into a plant expression vector (pMDC164) through gateway cloning. All four constructs were separately transformed into tobacco through Agrobacterium-mediated genetic transformation, and PCR-confirmed transgenic plants progressed to T-1 and T-2 generations. The T-2 transgenic tobacco plants comprising all PgHsp10pro fragments were used for GUS histochemical and qRT-PCR assays in different tissues under control and abiotic stresses. The PgHsp10PC pro expression was spedfic to stem and seedlings under control conditions. Under different abiotic stresses, particularly heat stress, PgHsp10PCpro had relatively higher activity than PgHsp10D1 pro, PgHsp10D2 pro and PgHsp10ASpro. PgHsp10pro from a stress resilient crop like pearl millet responds positively to a range of abiotic stresses, in particular heat, when expressed in heterologous plant systems such as tobacco. Hence, PgHsp10pro appears to be a potential promoter candidate for developing heat and drought stress-tolerant crop plants. (C) 2020 Published by Elsevier B.V.
机译：在本研究中，将珍珠米体热休克蛋白10（PGHSP10）基因的启动子区域进行并表征。 PGHSP10启动子（PGHSP10Pro）序列区域具有组织和非生物应激诱导性所需的所有顺式基序。通过网关克隆将完整的PGHSP10Pro（PGHSP10PC）和PGHSP10（PGHSP10D1和PGHSP10D2）的一系列PGHSP10（PGHSP10D1和PGHSP10D2）和反义形式的PGHSP10Pro（PGHSP10As）的截断。通过农杆菌介导的遗传转化分别将所有四种构建体分别转化为烟草，并且PCR确认的转基因植物进入T-1和T-2世代。包含所有PGHSP10Pro片段的T-2转基因烟草植物用于控制和非生物应力的不同组织中的GUS组织化学和QRT-PCR测定。 PGHSP10PC Pro表达在控制条件下被刺到茎和幼苗。在不同的非生物应力下，特别是热应激，PGHSP10PCPRO的活性比PGHSP10D1 Pro，PGHSP10D2 Pro和PGHSP10Aspro具有相对较高的活性。来自珍珠米的应力弹性作物的PGHSP10Pro，如珍珠米，当在烟草如烟草等异源植物系统中表达时，尤其是热量的一系列非生物应力。因此，PGHSP10PRO似乎是用于开发热和干旱胁迫性作物植物的潜在启动子候选者。 （c）2020由elsevier b.v发布。

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