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首页> 外文期刊>International Journal of Biological Macromolecules: Structure, Function and Interactions >Antitumor properties of a methyl-beta-D-galactopyranoside specific lectin from Kaempferia rotunda against Ehrlich ascites carcinoma cells
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Antitumor properties of a methyl-beta-D-galactopyranoside specific lectin from Kaempferia rotunda against Ehrlich ascites carcinoma cells

机译：来自kaempferia rotunda的甲基β-d-半乳糖醇特异性凝集素对Ehrlich腹水癌细胞的抗肿瘤性质

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摘要

A lectin was isolated from the tuberous rhizome of Keampferia rotunda by using different chromatographic methods with the molecular weight of 21 +/- 1 kDa. The lectin contained highest percentage of leucine and lowest percentage of tryptophan residues as determined by LC-MS. The lectin agglutinated mice and human erythrocytes and the hemagglutination activity was inhibited by Methyl-beta-D-galactopyranoside. The lectin did not lose its activity in the presence of urea but the activity abolished completely when treated with EDTA. The lectin exhibited its activity at the pH ranging from 6.0 to 9.0 and in a temperature range of 30-80 degrees C. Antiproliferative activity was studied against Ehrlich ascites carcinoma (EAC) and U87 cell lines. No inhibitory effect was observed against U87 cell line whereas 43.7% cell growth inhibition was observed in vitro against EAC cells at 160 mu g/ml. The lectin was injected (i.p.) in EAC bearing Swiss albino mice at the doses of 3.0 and 6.0 mg/kg/day for five consecutive days and 41 and 59% of EAC cell growth inhibition was observed, respectively. The cell growth inhibition was due to the induction of apoptosis in the EAC cells which was confirmed by cell morphological study, caspase-3 inhibitor and apoptosis-related gene expression. (C) 2017 Elsevier B.V. All rights reserved.
机译：通过使用不同的色谱方法使用不同的色谱方法，从旋转斑岩圆形大洲的结节根茎中分离凝集素，分子量为21 +/- 1kDa。凝集素含有最高百分比的亮氨酸和由LC-MS测定的色氨酸残留物的最低百分比。通过甲基β-D-吡喃吡喃糖苷抑制凝集素凝集的小鼠和人红细胞和血凝活性。凝集素在尿素存在下没有失去活性，但在用EDTA治疗时，活动完全废除的活动。凝集素在6.0至9.0的pH中表现出其活性，并且在30-80℃的温度范围内，研究了抗增殖活性对Ehrlich腹水癌（EAC）和U87细胞系进行了抗溶剂活性。对U87细胞系没有观察到抑制效果，而在160μg/ ml下在体外观察43.7％的细胞生长抑制。凝集素注射（I.P.）在EAC轴承的瑞士白化小鼠的3.0和6.0mg / kg /天的连续五天，分别观察到41和59％的EAC细胞生长抑制。细胞生长抑制是由于细胞形态学研究，Caspase-3抑制剂和凋亡相关基因表达确认的EAC细胞中凋亡的诱导。 （c）2017年Elsevier B.V.保留所有权利。

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