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首页> 外文期刊>International Journal of Biological Macromolecules: Structure, Function and Interactions >Evaluation of nanofibrous scaffolds obtained from blends of chitosan, gelatin and polycaprolactone for skin tissue engineering
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Evaluation of nanofibrous scaffolds obtained from blends of chitosan, gelatin and polycaprolactone for skin tissue engineering

机译：从壳聚糖，明胶和聚己内酯中获得的纳米纤维支架的评价，用于皮肤组织工程

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Polymer blending is a strategy commonly used to obtain hybrid materials possessing properties better than those of the individual constituents regarding their use in scaffolds for Tissue Engineering. In the present work, the scaffolds produced by electrospinning solutions of polymeric blends obtained using a polyester (polycaprolactone, PCL), a polysaccharide (chitosan, CS) and a protein (gelatin extracted from cold water fish skin, GEL), were investigated. Solutions conductivity, shear viscosity and surface tension were determined. GEL-containing scaffolds were crosslinked with vapour phase glutaraldehyde (GTA). The scaffolds were characterized physico-chemically regarding fibre morphology, porosity, water contact angle, mechanical properties, chemical bonds and fibre and dimensional stability upon immersion in water and cell culture medium, The scaffolds were further tested in vitro for cell adhesion, growth and morphology of human foetal fibroblasts (cell line HFFF2). Results show that the nanofibrous scaffolds are hydrophilic and display the typical porosity of non-woven fibre mats. The CS/PCL and CS/PCL/GEL scaffolds have the highest elastic modulus (48 MPa). Dimensional stability is best for the CS/PCL/GEL scaffolds. FTIR spectra confirm the occurrence of cross-linking reactions of GTA with both GEL and CS. Cell adhesion ratio ranked from excellent (close to 100%) to satisfactory (around 50%) in the order PCL/GEL > CS/GEL > CS/PCL/GEL > CS/PCL. Cell populations show an extended lag phase in comparison with the controls but cell proliferation occurs on all scaffolds until confluence is reached. In conclusion, all scaffolds studied possess characteristics that enable them to be used in skin tissue engineering but the CS/PCL/GEL scaffolds have better physical properties whereas the PCL/GEL scaffolds support a higher cell adhesion. (C) 2017 Elsevier B.V. All rights reserved.
机译：聚合物混合是一种常用的策略，用于获得具有比各个组分的性质的杂种材料更好地用于其在组织工程的支架中的使用。在本作工作中，研究了通过使用聚酯（聚己内酯，PCL），多糖（壳聚糖，Cs）和蛋白质（从冷水鱼皮，凝胶中提取的明胶）获得的聚合物共混物的静电纺丝溶液产生的支架。解决方案导电性，剪切粘度和表面张力。含凝胶的支架与气相戊二醛（GTA）交联。在浸入水和细胞培养基时，在水化学上表征了对纤维形态，孔隙率，水接触角，机械性能，化学键和纤维和尺寸稳定性的支架。在体外进行细胞粘附，生长和形态，进一步测试支架在水和细胞培养基中。人胎成纤维细胞（细胞系HFFF2）。结果表明，纳米纤维支架是亲水性的，显示非织造纤维垫的典型孔隙率。 CS / PCL和CS / PCL /凝胶支架具有最高的弹性模量（48MPa）。尺寸稳定性最适合CS / PCL /凝胶支架。 FTIR光谱通过凝胶和Cs确认GTA交联反应的发生。在顺序PCL /凝胶> Cs /凝胶> Cs / PCL /凝胶> CS / PCL中，细胞粘附比以优异的（接近100％）排名至令人满意的（约50％）。细胞群显示与对照相比的延长滞后阶段，但在所有支架上发生细胞增殖，直到达到汇合。总之，研究的所有支架都具有使它们在皮肤组织工程中使用的特性，但CS / PCL /凝胶支架具有更好的物理性质，而PCL /凝胶支架支持更高的细胞粘附性。 （c）2017年Elsevier B.V.保留所有权利。

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