...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>International Journal of Biological Macromolecules: Structure, Function and Interactions >Interaction between chitosan and its related enzymes: A review
【24h】

Interaction between chitosan and its related enzymes: A review

机译：壳聚糖与其相关酶之间的相互作用：综述

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Chitosan-related enzymes including chitosanases, exo-beta-glucosaminidases, and enzymes having chitosan-binding modules recognize ligands through electrostatic interactions between the acidic amino acids in proteins and amino groups of chitosan polysaccharides. However, in GH8 chitosanases, several aromatic residues are also involved in substrate recognition through stacking interactions, and these enzymes consequently hydrolyze beta-1,4-glucan as well as chitosan. The binding grooves of these chitosanases are extended and opened at both ends of the grooves, so that the enzymes can clamp a long chitosan polysaccharide. The association/dissociation of positively charged glucosamine residues to/from the binding pocket of a GH2 exo-beta-glucosaminidase controls the p K-a of the catalytic acid, thereby maintaining the high catalytic potency of the enzyme. In contrast to chitosanases, chitosan-binding modules only accommodate a couple of glucosamine residues, predominantly recognizing the non-reducing end glucosamine residue of chitosan by electrostatic interactions and a hydrogen-bonding network. These structural findings on chitosan-related enzymes may contribute to future applications for the efficient conversion of the chitin/chitosan biomass. (C) 2017 Elsevier B.V. All rights reserved.
机译：壳聚糖相关酶包括壳聚糖酶，外β-葡糖胺酶和具有壳聚糖结合模块的酶，识别配体，通过蛋白质和壳聚糖多糖的蛋白质和氨基中的酸性氨基酸之间的静电相互作用。然而，在GH8壳聚糖酶中，几种芳族残基也通过堆叠相互作用参与底物识别，因此这些酶因此水解β-1,4-葡聚糖以及壳聚糖。这些壳聚糖酶的结合槽在凹槽的两端延伸并打开，使得酶可以夹紧长的壳聚糖多糖。带正电荷的葡糖胺残基与GH2外β-葡糖胺酶的结合袋的关联/解离对照催化酸的P k-A，从而保持酶的高催化效力。与壳聚糖酶相比，壳聚糖结合模块仅适应几种葡糖胺残基，主要通过静电相互作用和氢键网络识别壳聚糖的非还原末端葡糖胺残留物。对壳聚糖相关酶的这些结构发现可能有助于未来的壳蛋白/壳聚糖生物质的有效转化的应用。 （c）2017年Elsevier B.V.保留所有权利。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号