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首页> 外文期刊>International Journal of Biological Macromolecules: Structure, Function and Interactions >Alginate enhances Toll-like receptor 4-mediated phagocytosis by murine RAW264.7 macrophages
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Alginate enhances Toll-like receptor 4-mediated phagocytosis by murine RAW264.7 macrophages

机译：藻酸盐增强了鼠Raw264.7巨噬细胞的Toll样受体4介导的吞噬作用

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摘要

Alginate is a naturally acidic polysaccharide consisting alternately of beta-D-mannuronic acid and alpha-L-guluronic acid with 1, 4-glycosidic linkages and is derived from brown seaweeds. Herein, the effect of alginate on the promotion of macrophage phagocytosis and the corresponding molecular mechanisms were investigated in murine RAW264.7 cells. Alginate could enhance the intracellular phagocytosis of gold nanoparticles (AuNPs), fluorescent microspheres and immunoglobulin G (IgG)-opsonized Staphylococcus aureus (S. aureus). Moreover, alginate increased Toll-like receptor 4 (TLR4) expression and activated the Akt/nuclear factor-kappa B (NF-kappa B) and p38 mitogen-activated protein kinase (MAPK) signalling pathways. Alginate-promoted phagocytosis was suppressed by the addition of inhibitors of TLR4, NF-kappa B and p38 MAPK and by TLR4 gene knockdown, indicating the involvement of these key components. This work is the first to propose that alginate promotes phagocytosis via upregulating TLR4 expression and stimulating the Akt/NF-kappa B and p38 MAPK signalling pathways, which may contribute to the capacity of alginate to activate macrophages. (C) 2017 Published by Elsevier B.V.
机译：藻酸盐是具有1,4糖苷键组成的β-d甘露糖醛酸和α-L-古洛糖醛酸的交替的天然酸性多糖，并从褐海藻衍生的。在本文中，藻酸盐对促进巨噬细胞吞噬的和相应的分子机制作用在小鼠RAW264.7细胞进行了研究。藻酸盐可以增强金粒子（AuNPs），荧光微球的细胞内吞噬作用和免疫球蛋白G（IgG）的-opsonized金黄色葡萄球菌（金黄色葡萄球菌）。此外，藻增加Toll样受体4（TLR4）的表达和活化的Akt的/核因子-κB（NF-κB的）和信号通路p38促分裂原活化蛋白激酶（MAPK）。藻酸盐促进的吞噬作用，通过加入TLR4，NF-κB的抑制剂以及p38蛋白，并通过TLR4基因敲低抑制，这表明这些关键部件的参与。这项工作是最早提出的是藻酸盐经由上调TLR4表达并刺激的Akt / NF-κB与p38蛋白信号传导途径，其可以有助于藻酸盐来激活巨噬细胞的能力促进吞噬作用。 （c）2017年由Elsevier B.V发布。

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  • 作者

    Bi Decheng; Zhou Rui; Cai Nan; Lai Qiuxian; Han Qingguo; Peng Yanwen; Jiang Zedong; Tang Zhishu; Lu Jun; Bao Weiyang; Xu Hong; Xu Xu;

  • 作者单位

    Shenzhen Univ Shenzhen Key Lab Marine Bioresources &

    Ecol Coll Life Sci &

    Oceanog Shenzhen 518060 Peoples R China;

    Shenzhen Univ Shenzhen Key Lab Marine Bioresources &

    Ecol Coll Life Sci &

    Oceanog Shenzhen 518060 Peoples R China;

    Shenzhen Univ Shenzhen Key Lab Marine Bioresources &

    Ecol Coll Life Sci &

    Oceanog Shenzhen 518060 Peoples R China;

    Shenzhen Univ Shenzhen Key Lab Marine Bioresources &

    Ecol Coll Life Sci &

    Oceanog Shenzhen 518060 Peoples R China;

    Shenzhen Univ Shenzhen Key Lab Marine Bioresources &

    Ecol Coll Life Sci &

    Oceanog Shenzhen 518060 Peoples R China;

    Sun Yat Sen Univ Affiliated Hosp 3 Cell Gene Therapy Translat Med Res Ctr Guangzhou 510630 Guangdong Peoples R China;

    Jimei Univ Fujian Prov Key Lab Food Microbiol &

    Enzyme Engn Xiamen 361021 Peoples R China;

    Shaanxi Univ Chinese Med Shaanxi Prov Key Lab New Drugs Shaanxi Collaborat Innovat Ctr Chinese Med Resour Xianyang 712046 Peoples R China;

    Shenzhen Univ Shenzhen Key Lab Marine Bioresources &

    Ecol Coll Life Sci &

    Oceanog Shenzhen 518060 Peoples R China;

    Yangzhou Univ Coll Environm Sci &

    Engn Yangzhou 225009 Jiangsu Peoples R China;

    Shenzhen Univ Shenzhen Key Lab Marine Bioresources &

    Ecol Coll Life Sci &

    Oceanog Shenzhen 518060 Peoples R China;

    Shenzhen Univ Shenzhen Key Lab Marine Bioresources &

    Ecol Coll Life Sci &

    Oceanog Shenzhen 518060 Peoples R China;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 生物大分子的结构和功能;
  • 关键词

    Alginate; Macrophage; Phagocytosis; Toll-like receptor 4;

    机译：藻酸盐;巨噬细胞;吞噬作用;达洛般的受体4;

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