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首页> 外文期刊>Electrophoresis: The Official Journal of the International Electrophoresis Society >Enantioseparation of hydroxyeicosatetraenoic acids by hydroxypropyl‐γ‐cyclodextrin‐modified micellar electrokinetic chromatography
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Enantioseparation of hydroxyeicosatetraenoic acids by hydroxypropyl‐γ‐cyclodextrin‐modified micellar electrokinetic chromatography

机译：羟丙基-γ-环糊精改性胶束电动色谱法对羟基辛酸四烯酸进行映对分析

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摘要

Complete resolution of hydroxyeicosatetraenoic acid (HETE) enantiomers was achieved using hydroxypropyl‐γ‐cyclodextrin (HP‐γ‐CD)‐modified MEKC. The optimum running conditions were determined to be utilizing a 30 mM phosphate–15 mM borate buffer (pH 9.0) containing 30 mM HP‐γ‐CD and 75 mM SDS as the BGE, application of +30 kV as the effective voltage, and carrying out the experiment at 15°C. The eluents were detected at 235 nm. The method was used successfully for the simultaneous separations of ( S )‐ and ( R )‐enantiomers of regioisomeric 8‐, 11‐, 12‐, and 15‐HETEs. Subsequently, the optimized method was applied to evaluate the stereochemistry of 8‐ and 12‐HETEs from the marine red algae, Gracilaria vermiculophylla and Gracilaria arcuata , respectively. The 8‐HETE was found to be a mixture of 98% ( R )‐enantiomer and 2% ( S )‐enantiomer, while the 12‐HETE was a mixture of 98% ( S )‐enantiomer and 2% ( R )‐enantiomer. The present study demonstrates that the HP‐γ‐CD‐modified MEKC method is simple and sensitive and provides unambiguous information on the configuration of natural and synthetic HETEs.
机译：使用羟丙基-γ-环糊精（HP-γ-CD） - 制定的MEKC实现完全分离羟基辛辛醚酸（HETE）对映异构体。确定最佳运行条件利用含有30mM HP-γ-Cd和75mm SDS的30mM磷酸盐-15mM硼酸硼缓冲液（pH9.0）作为BGE，施加+30kV作为有效电压，并携带在15°C下实验。洗脱液在235nm处检测到。该方法成功地用于同时分离（S） - 和（R） - 丙烷橡皮聚体的同时分离8-，11-，12-和15-Hetes。随后，应用了优化的方法，评价了海洋红藻类，Gracilaria蛭糊糊剂和Gracilaria Arcuata的8-和12-Hetes的立体化学。发现8-HETE是98％（R） - 托体和2％ - 蒽酸的混合物，而12-HETE是98％ - 托体团和2％（R）的混合物 - 对映体。本研究表明，HP-γ-CD修改的MEKC方法简单且敏感，并提供有关自然和合成Het的配置的明确信息。

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