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首页> 外文期刊>Environmental Science and Pollution Research >The effect of anaerobic-aerobic and feast-famine cultivation pattern on bacterial diversity during poly-beta-hydroxybutyrate production from domestic sewage sludge
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The effect of anaerobic-aerobic and feast-famine cultivation pattern on bacterial diversity during poly-beta-hydroxybutyrate production from domestic sewage sludge

机译：厌氧 - 有氧饮食栽培模式对家庭污水污泥多β-羟基丁酯生产中细菌多样性的影响

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摘要

The main objective of this work was to investigate the influence of different oxygen supply patterns on poly-beta-hydroxybutyrate (PHB) yield and bacterial community diversity. The anaerobic-aerobic (A/O) sequencing batch reactors (SBR1) and feast-famine (F/F) SBR2 were used to cultivate activated sludge to produce PHB. The mixed microbial communities were collected and analyzed after 3 months cultivation. The PHB maximum yield was 64 wt% in SBR1 and 53 wt% in SBR2. Pyrosequencing analysis 16S rRNA gene of two microbial communities indicated there were nine and four bacterial phyla in SBR1 and SBR2, respectively. Specifically, Proteobacteria (36.4% of the total bacterial community), Actinobacteria (19.7 %), Acidobacteria (14.1 %), Firmicutes (4.4 %), Bacteroidetes (1.7 %), Cyanobacteria/Chloroplast (1.5 %), TM7 (0.8 %), Gemmatimonadetes (0.2 %), and Nitrospirae (0.1 %) were present in SBR1. Proteobacteria (94.2 %), Bacteroidetes (2.9 %), Firmicutes (1.9 %), and Actinobacteria (0.7 %) were present in SBR2. Our results indicated the SBR1 fermentation system was more stable than that of SBR2 for PHB accumulation.
机译：这项工作的主要目的是探讨不同氧气供给模式对多β-羟基丁酯（PHB）产量和细菌群落多样性的影响。使用厌氧 - 有氧（A / O）测序批量反应器（SBR1）和饲料 - 饥荒（F / F）SBR2用于培养活性污泥以产生PHB。在3个月培养后收集并分析混合的微生物群落。在SBR1和53wt％中，PHB最大收率为64wt％，在SBR2中。焦磷酸盐分析16S分别表明两种微生物群落的RRNA基因分别在SBR1和SBR2中存在九个和四种细菌植物。具体而言，植物聚糖（占总细菌群落的36.4％），抗菌菌（19.7％），抗酸杆菌（14.1％），压骨细菌（4.4％），细胞（1.7％），蓝藻/叶绿体（1.5％），TM7（0.8％） ，GemmatimonAdetes（0.2％）和氮素（0.1％）存在于SBR1中。在SBR2中存在植物聚糖（94.2％），细菌（2.9％），压缩（1.9％）和肌动菌（0.7％）。我们的结果表明，SBR1发酵系统比SBR2更稳定，用于PHB积累。

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