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首页> 外文期刊>Environmental Science and Pollution Research >Biogenic synthesis of silver nanoparticle by using secondary metabolites from Pseudomonas aeruginosa DM1 and its anti-algal effect on Chlorella vulgaris and Chlorella pyrenoidosa
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Biogenic synthesis of silver nanoparticle by using secondary metabolites from Pseudomonas aeruginosa DM1 and its anti-algal effect on Chlorella vulgaris and Chlorella pyrenoidosa

机译：铜绿假单胞菌DM1中次代谢物的生物合成银纳米粒子及其对小球藻和小球藻菌抗藻类效果

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摘要

Biogenic synthesis of silver nanoparticles (AgNPs) using extracellular metabolites from the bacterium Pseudomonas aeruginosa DM1 offers an eco-friendly and sustainable way of metal nanoparticle synthesis. The present work highlights the biotransformation of silver nitrate solution into AgNP, mediated by extracellular secondary metabolite pyoverdine, a siderophore produced by P. aeruginosa. The bioreduction of silver ions into AgNPs by using pyoverdine was recorded in terms of Fourier transform infrared spectroscopy (FTIR) analysis and color change in the reaction mixture (AgNO3 + pyoverdine) from pale yellow to dark brown with absorption maxima at 415 nm. The results of X-ray diffraction (XRD) analysis of AgNPs showed its crystalline face-centered cubic structure. The scanning electron microscopy (SEM) and transmission electron microscopy (TEM) pictures of AgNPs showed spherical morphology of AgNP in the range of 45100 nm, with tendency of agglomerations. The energy-dispersive X- ray (EDX) analysis of particles provided strong signal of elemental silver with few minor peaks of other impurities. The present approach offers a unique in vitro method of metal nanoparticle synthesis by exogenously produced bacterial secondary metabolites, where direct contact between the toxic metal and biological resource material can be avoided. The biologically synthesized AgNPs are found to have antialgal effects against two species of Chlorella (Chlorella vulgaris and Chlorella pyenoidosa), as indicated by zone of growth inhibition on algal culture plates. Further results exhibit concentration-dependent progressive inhibition of chlorophyll content in the algal cells by AgNPs, confirming the algicidal effect of AgNPs.
机译：使用来自铜绿假单胞菌的细胞外代谢物的银纳米颗粒（AgNP）的生物合成提供了一种环保和可持续的金属纳米粒子合成方式。本作本作突出了硝酸银溶液中的生物转化，该溶液中的细胞外次级代谢物钙晶岩介导，由P.铜绿假单胞菌生产的阳光。通过傅里叶变换红外光谱（FTIR）分析和从浅黄色至深棕色的反应混合物（AgNO3 +百voverdine）的颜色变化，在415nm的吸收最大值中的反应混合物（AgNo3 +百voverdine）中的颜色变化和颜色变化，将银离子对AgNP的生物切换和使用棕色的变化。 AgNP的X射线衍射（XRD）分析的结果显示其晶体面为中心的立方结构。扫描电子显微镜（SEM）和透射电子显微镜（TEM）AgNP的图片显示为45100nm范围内AgNP的球形形态，具有附聚的趋势。颗粒的能量分散X射线（EDX）分析提供了几个杂质的细胞银信号的强信号。本方法通过外源生产的细菌次级代谢物提供了一种独特的金属纳米粒子合成方法，其中可以避免毒性金属和生物资源材料之间的直接接触。发现生物合成的agnps对两种小球藻（小球藻和小球藻氏蛋白气体）具有抗高速效应，如在藻类培养板上的生长抑制区所示。进一步的结果，通过AgNP表现出藻类细胞中叶绿素含量的浓度依赖性逐变抑制，证实了AgNP的杀虫作用。

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