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首页> 外文期刊>International journal of nanomechanics science and technology >COLLECTION, ISOLATION,IDENTIFICATION, AND BIOSYNTHESIS OF SILVER NANOPARTICLES USING MICROALGA CHLORELLA PYRENOIDOSA
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COLLECTION, ISOLATION,IDENTIFICATION, AND BIOSYNTHESIS OF SILVER NANOPARTICLES USING MICROALGA CHLORELLA PYRENOIDOSA

机译：微叶藻小球藻的银纳米颗粒的收集，分离，鉴定和生物合成

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The samples were collected from rock ponds in Kovalam, isolated with the aid of plating techniques, and identified at the Krishnamurthy Institute of Algology, Chennai. Algal cells were grown in a Bolds Basal medium and cultured for 14 days for 16:8 h at 24°C. In 14 days the algal cells were pelleted by centrifugation. The bioreduction of Ag~+ ions in solution was monitored by means of the UV-visibk spectroscopy from zero reading, and doubly distilled water was used as a blank. The samples were withdrawn at various time intervals and the absorbance was measured at 450 nm. The silver nanoparticles synthesized using extracts of algal samples was confirmed by color changes and was characterized by a UV-visible spectrophotometer; the UV-visible spectra showed a broad peak located at 450 nm for silver nanoparticles. The TEM analysis shows small spherical 5-10-nm-sized particles. The nanoparticles were also characterized by the SEM, EDAX, XRD, and FTIR analysis.
机译：样品是从科瓦兰（Kovalam）的石塘中收集的，借助电镀技术进行分离，并在钦奈的克里希那穆提（Krishnamurthy）法学院进行了鉴定。藻类细胞在Bolds Basal培养基中生长，并在24°C下培养14天16：8 h。在14天内，通过离心沉淀藻类细胞。通过UV-可见光谱从零读数监测溶液中Ag +离子的生物还原，并将双蒸馏水用作空白。以不同的时间间隔取出样品，并在450nm处测量吸光度。通过颜色变化证实了使用藻类样品提取物合成的银纳米颗粒，并通过紫外可见分光光度计对其进行了表征。紫外可见光谱显示银纳米颗粒位于450 nm处的宽峰。 TEM分析显示5-10nm球形小颗粒。纳米颗粒还通过SEM，EDAX，XRD和FTIR分析表征。

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