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Electrochemiluminescent detection of Escherichia coli O157:H7 based on Ru(bpy)(3)(2+)/ZnO nanorod arrays

机译:基于Ru(BPY)(3)(2 +)/ ZnO纳米棒阵列的Escherichia Coli O157:H7的电化学发光检测

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摘要

Foodbome pathogens are perpetual threats to human and animal health. Detection of pathogens requires accurate, sensitive, rapid and point-of-care diagnostic assays. In this study, we described a simple and sensitive electrochemiluminescent (ECL) assay to detect the deadly bacteria Escherichia coli O157:H7 by Ru(bpy)(3)(2+)-coated ZnO nanorods arrays (NAs). The Ru(bpy)(3)(2+)-coated ZnO NAs were fabricated by immobilizing Ru(bpy)(3)(2+) on ZnO NAs with a large specific surface area and good conductivity. An Ru(bpy)(3)(2+)-2-(dibutylamino)-ethanol (DBAE) system coated on ZnO NAs exhibits high ECL intensity, rapid response and good stability. This system was further developed as an ECL immunosensor used in the detection of E. coli O157:H7. The proposed ECL immunosensor exhibits a broad detection range within the scope of 200-100 000 CFU ml(-1) and quite a low detection limit of 143 CFU ml(-1). The high specificity, remarkable reproducibility and good stability offer a sensitive, selective, and convenient pathway for detecting E. coli O157:H7 in the field of food safety and clinical diagnosis.
机译:食物组群病原体是人类和动物健康的永久威胁。病原体检测需要准确,灵敏,快速和护理点诊断测定。在该研究中,我们描述了一种简单敏感的电化学发光(ECL)测定以检测致命的细菌大肠杆菌O157:H7通过Ru(BPY)(3)(3)(2 +)涂覆的ZnO纳米峰阵列(NAS)。通过在具有大的比表面积和良好的导电性的ZnO NA上固定Ru(BPY)(3)(3)(2+)来制造Ru(BPY)(3)(2 +)涂覆的ZnO NIS。涂覆在ZnO NAS上的Ru(BPY)(3)(3)(2 +) - 2-(二丁基氨基) - 乙醇(DBAE)系统表现出高ECL强度,快速响应和良好稳定性。该系统进一步开发为用于检测大肠杆菌O157:H7的ECL免疫传感器。所提出的ECL免疫传感器在200-100000cfu ml(-1)范围内具有宽的检测范围,并且具有相当低的检测限为143 cfu ml(-1)。高特异性,显着的再现性和良好的稳定性为检测食品安全和临床诊断领域的大肠杆菌O157:H7提供敏感,选择性和方便的途径。

著录项

  • 来源
    《Nanotechnology》 |2019年第2期|共7页
  • 作者单位

    Harbin Univ Sci &

    Technol Sch Chem &

    Environm Engn Harbin 150040 Heilongjiang Peoples R China;

    Harbin Inst Technol Micro &

    Nanotechnol Res Ctr Harbin 150080 Heilongjiang Peoples R China;

    Harbin Inst Technol Condensed Matter Sci &

    Technol Inst Dept Phys Harbin 150080 Heilongjiang Peoples R China;

    Harbin Univ Sci &

    Technol Sch Chem &

    Environm Engn Harbin 150040 Heilongjiang Peoples R China;

    Harbin Univ Sci &

    Technol Sch Chem &

    Environm Engn Harbin 150040 Heilongjiang Peoples R China;

    Harbin Univ Sci &

    Technol Sch Chem &

    Environm Engn Harbin 150040 Heilongjiang Peoples R China;

    Harbin Univ Sci &

    Technol Sch Chem &

    Environm Engn Harbin 150040 Heilongjiang Peoples R China;

    Harbin Inst Technol Condensed Matter Sci &

    Technol Inst Dept Phys Harbin 150080 Heilongjiang Peoples R China;

    Harbin Inst Technol Micro &

    Nanotechnol Res Ctr Harbin 150080 Heilongjiang Peoples R China;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 特种结构材料;
  • 关键词

    ZnO nanorod arrays; electrochemiluminescent detection; E. coli O157 H7; Ru(bpy)(3)(2+);

    机译:ZnO Nanorod阵列;电化学发光检测;e。 Coli O157 H7;Ru(BPY)(3)(2+);

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