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Targeted gold nanorods combined with low-intensity nsPEFs enhance antimelanoma efficacyin vitro

机译：有针对性的金纳米棒与低强度nspefs联合增强抗身效效应体外

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摘要

High-intensity nanosecond pulsed electric fields (nsPEFs) are a novel treatment with promising applications for cell stimulation and tissue ablation, and many research studies have shown that gold nanorods (GNRs) are high-conductivity nanomaterials that can enhance electroporation for biomedical applications. In addition, the folic acid (FA) receptor has been demonstrated as a valuable therapeutic target that is highly expressed in a variety of cancers. To reduce the electric field strength required to treat tumors by nsPEFs, for the first time, gold nanorods with folic acid were proposed to achieve higher antimelanoma efficacy at lower electric field intensity in this study. The surface of polyethylene glycol-gold nanorods with good biocompatibility was further modified by folic acid (FA) to provide modified gold nanorods (GNR-PEG-FA) with specific targeted recognition of A375 melanoma cells. The binding of GNRs to A375 melanoma cells was observed by dark field microscopy. After combined treatment with nsPEFs and GNRs, cell viability was evaluated by a CCK-8 assay. Flow cytometry was performed to evaluate apoptosis and the cell cycle. And active caspase 3 was also detected after treatment. The antimelanoma efficacy was enhanced in a pulsed electric field-dependent manner. More importantly, compared with the group of nsPEFs alone and gold nanorods without FA, treating cells with nsPEFs combined with GNR-PEG-FA resulted in a lower percentage of viable cells, higher percentages of necrosis and apoptosis and higher concentration of active caspase 3 and induced cell cycle arrest in S phase, effectively inhibiting the proliferation of A375 melanoma cells. nsPEFs combined with GNR-PEG-FA showed the best antimelanoma efficacyin vitroand effectively killed melanoma cells with low-intensity nsPEFs. The combined treatment of cells with nsPEFs and GNR-PEG-FA is expected to become a safer and more efficient physical treatment of melanomas.
机译：高强度纳秒脉冲电场（NPEFS）是一种新颖的处理，具有对细胞刺激和组织消融的有希望应用，并且许多研究研究表明，金纳米棒（GNR）是高导电纳米材料，可以增强生物医学应用的电穿孔。此外，已经证明了叶酸（Fa）受体作为在各种癌症中高表达的有价值的治疗靶标。为了减少NPEFS治疗肿瘤所需的电场强度，首次提出具有叶酸的金纳米棒，以在该研究中以较低的电场强度实现更高的抗身肿瘤效果。通过叶酸（Fa）进一步修饰具有良好生物相容性的聚乙二醇 - 金纳米棒的表面，以提供具有特异性靶向识别A375黑色素瘤细胞的改性金纳米棒（GNR-PEG-FA）。通过暗场显微镜观察GNR对A375黑色素瘤细胞的结合。在用NPEFS和GNR组合处理后，通过CCK-8测定评估细胞活力。进行流式细胞术以评估细胞凋亡和细胞周期。处理后也检测到活性胱天悬浮酶3。以脉冲电场依赖性方式增强了抗身疗效。更重要的是，与单独的NPEFS组和没有FA的金纳米棒进行比较，用NPEFS与GNR-PEG-FA合并的细胞导致可活细胞的较低百分比，更高的坏死百分比和凋亡百分比和更高浓度的活性胱天蛋白酶3诱导细胞周期停滞S期，有效抑制A375黑素瘤细胞的增殖。 NPSPEF与GNR-PEG-FA相结合，显示了最佳的抗身疗效培体培体有效杀死具有低强度NPSPEF的黑色素瘤细胞。预期使用NPEFS和GNR-PEG-FA细胞的组合治疗成为Melanomas的更安全和更有效的物理治疗。

著录项

  • 来源
    《Nanotechnology》 |2020年第35期|共11页
  • 作者

    Mi Yan; Liu Quan; Li Pan; Xu Jin; Yang Qiyu; Tang Junying;

  • 作者单位

    Chongqing Univ State Key Lab Power Transmiss Equipment &

    Syst Se Chongqing 400044 Peoples R China;

    Chongqing Univ State Key Lab Power Transmiss Equipment &

    Syst Se Chongqing 400044 Peoples R China;

    Chongqing Univ State Key Lab Power Transmiss Equipment &

    Syst Se Chongqing 400044 Peoples R China;

    Chongqing Univ State Key Lab Power Transmiss Equipment &

    Syst Se Chongqing 400044 Peoples R China;

    Chongqing Med Sci Univ Affiliated Hosp 1 Chongqing 400044 Peoples R China;

    Chongqing Med Sci Univ Affiliated Hosp 1 Chongqing 400044 Peoples R China;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 特种结构材料;
  • 关键词

    nanosecond pulsed electric fields; gold nanorods; cell viability; apoptosis; cell cycle;

    机译：纳秒脉冲电场;金纳米棒;细胞活力;细胞凋亡;细胞周期;

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