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首页> 外文期刊>Nanotechnology >Influence of medium viscosity and intracellular environment on the magnetization of superparamagnetic nanoparticles in silk fibroin solutions and 3T3 mouse fibroblast cell cultures
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Influence of medium viscosity and intracellular environment on the magnetization of superparamagnetic nanoparticles in silk fibroin solutions and 3T3 mouse fibroblast cell cultures

机译：中粘剂和细胞内环境对丝纤维蛋白溶液超顺磁性纳米粒子磁化的影响及3T3小鼠成纤维细胞培养物

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Biomedical applications based on the magnetic properties of superparamagnetic iron oxide nanoparticles (SPIONs) may be altered by the mechanical attachment or cellular uptake of these nanoparticles. When nanoparticles interact with living cells, they are captured and internalized into intracellular compartments. Consequently, the magnetic behavior of the nanoparticles is modified. In this paper, we investigated the change in the magnetic response of 14 nm magnetic nanoparticles (Fe3O4) in different solutions, both as a stable liquid suspension (one of them mimicking the cellular cytoplasm) and when associated with cells. The field-dependent magnetization curves from inert fluids and cell cultures were determined by using an alternating gradient magnetometer, MicroMagTM 2900. The equipment was adapted to measure liquid samples because it was originally designed only for solids. In order to achieve this goal, custom sample holders were manufactured. Likewise, the nuclear magnetic relaxation dispersion profiles for the inert fluid were also measured by fast field cycling nuclear magnetic relaxation relaxometry. The results show that SPION magnetization in inert fluids was affected by the carrier liquid viscosity and the concentration. In cell cultures, the mechanical attachment or confinement of the SPIONs inside the cells accounted for the change in the dynamic magnetic behavior of the nanoparticles. Nevertheless, the magnetization value in the cell cultures was slightly lower than that of the fluid simulating the viscosity of cytoplasm, suggesting that magnetization loss was not only due to medium viscosity but also to a reduction in the mechanical degrees of freedom of SPIONs rotation and translation inside cells. The findings presented here provide information on the loss of magnetic properties when nanoparticles are suspended in viscous fluids or internalized in cells. This information could be exploited to improve biomedical applications based on magnetic propert
机译：基于超顺磁性氧化铁纳米粒子（栓塞）的磁性的生物医学应用可以通过这些纳米颗粒的机械连接或蜂窝摄取来改变。当纳米颗粒与活细胞相互作用时，它们被捕获并内化成细胞内隔室。因此，修饰纳米颗粒的磁性行为。在本文中，我们研究了不同溶液中14nm磁性纳米粒子（Fe3O4）的磁响应的变化，既是稳定的液体悬浮液（其中一个模拟细胞细胞质）和与细胞相关时。通过使用交替梯度磁力计，MicroMagtm 2900来测定来自惰性流体和细胞培养物的现场磁化曲线。该设备适于测量液体样品，因为它最初仅设计用于固体。为了实现这一目标，制造定制样品架。同样地，通过快速场循环核磁弛豫弛豫测量，还测量惰性流体的核磁弛豫分散型材。结果表明，惰性流体中的SpiON磁化受载流液粘度和浓度的影响。在细胞培养物中，细胞内部的熔接的机械连接或限制占纳米颗粒的动态磁性行为的变化。然而，细胞培养物中的磁化值略低于模拟细胞质粘度的流体的磁化值，这表明磁化损失不仅是由于中等粘度，而且还可以减少散热的机械自由度的减少内部细胞。这里提出的发现提供了有关纳米颗粒悬浮在粘性流体中或在细胞内部化的磁性损失的信息。可以利用此信息来改进基于磁性的生物医学应用程序

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