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首页> 外文期刊>Life sciences >Effect of Lactobacillus plantarum and L-arginine against endotoxin-induced liver injury in a rat model.
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Effect of Lactobacillus plantarum and L-arginine against endotoxin-induced liver injury in a rat model.

机译：Lactobacillus plantarum和L-精氨酸对大鼠内毒素诱导的肝损伤的影响。

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AIMS: Use of probiotics, alone or as adjunct to other established therapies, has been reported to have potential benefits. Recently, we have reported protective potential of probiotic against Salmonella-induced liver injury. However, co-supplementation with prebiotics did not result in meaningful synergism at systemic level. Owing to the action of probiotics at the mucosal level and of arginine at systemic level, the present study was designed to evaluate the effect of Lactobacillus plantarum alone or in conjunction with arginine to combat endotoxin-mediated liver injury in rats. MAIN METHODS: Bacterial endotoxin/lipopolysaccharide (LPS) was injected intraperitoneally and animals were sacrificed 8h post-challenge. Efficacy of L. plantarum alone or in conjunction with l-arginine was determined on the basis of enzyme markers, histology, levels of nitric oxide (NO) and tumor necrosis factor-alpha (TNF-alpha) in addition to identification of amino acids by paper chromatography. KEY FINDINGS: Prior supplementation of LPS-challenged rats with L. plantarum (10(10)CFU per rat given orally for 10 days) demonstrated decreased levels of liver enzymes, NO and TNF-alpha. Interestingly, complementing Lactobacillus with arginine revealed a synergistic decrease not only in the liver markers but also in NO and TNF-alpha along with increased intensity of ornithine and methionine. Histological evidence also confirmed the protective efficacy of probiotic in conjunction with l-arginine. SIGNIFICANCE: Presence of ornithine and methionine in the probiotic-arginine co-supplemented group suggests involvement of arginase-induced synthesis of polyamines. This study highlights that L. plantarum may direct l-arginine metabolism towards polyamine synthesis thereby exhibiting synergistic effect against liver injury.
机译：目的：据报道，使用益生菌或作为其他既定疗法的辅助使用潜在的益处。最近，我们已经报道了益生菌对沙门氏菌诱导的肝损伤的保护潜力。然而，与益生元的共同补充并没有导致系统性水平有意义的协同作用。由于益生菌的作用在粘膜水平和全身水平的精氨酸，本研究旨在评估单独的乳酸杆菌植物体或与精氨酸结合对抗内毒素介导的大鼠肝损伤的影响。主要方法：腹膜内注射细菌内毒素/脂多糖（LPS），攻击后8小时内处死动物。除了鉴定氨基酸之外，基于酶标记，组织学，一氧化氮（NO）和肿瘤坏死因子-α（TNF-α），确定L.Portararum单独或与L-精氨酸结合的疗效。除了氨基酸外纸张色谱。主要发现：在口服L. plastarum（10（10）CFU 10天给定10天的10（10）CFU）的提前补充，证明了肝酶，NO和TNF-α的降低。有趣的是，与精氨酸的乳酸杆菌相互补充，不仅在肝脏标记中揭示了协同减少，而且没有在NO和TNF-α以及鸟氨酸和蛋氨酸的增加和TNF-α。组织学证据还证实了益生菌与L-精氨酸结合的保护效果。意义：益生菌 - 精氨酸共同补充基团中的鸟氨酸和蛋氨酸的存在表明氨基酶诱导的多胺的合成涉及。该研究突出显示L.Platarum可以将L-精氨酸代谢指向多胺合成，从而表现出对肝损伤的协同效应。

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