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首页> 外文期刊>Life sciences >WNT/beta-catenin pathway mediates the anti-adipogenic effect of platycodin D, a natural compound found in Platycodon grandiflorum.
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WNT/beta-catenin pathway mediates the anti-adipogenic effect of platycodin D, a natural compound found in Platycodon grandiflorum.

机译：Wnt / beta-catenin途径介导Platycodin D，在Platycodon Grandiflorum中发现的天然化合物的抗脂肪蛋白效果。

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AIMS: This study was conducted to suggest the role of WNT/beta-catenin pathway in the anti-adipogenic effect of platycodin D, a natural compound found in Platycodon grandiflorum. MAIN METHODS: Gene knockdown experiments using small interfering RNA (siRNA) transfection were conducted to elucidate crucial role of beta-catenin in the anti-adipogenic effects of platycodin D. Real-Time PCR and Western blot were used to analyze the expression levels of mRNAs and proteins in the WNT/beta-catenin pathway. KEY FINDINGS: During the adipocyte differentiation of 3 T3-L1 cells, members of the WNT/beta-catenin pathway were normally down-regulated, whereas platycodin D significantly reinstated the WNT/beta-catenin pathway. The mRNA and protein expressions of disheveled (DVL) 2, which stabilize beta-catenin, were increased by platycodin D treatment, but the protein level of AXIN, which induces the degradation of beta-catenin, was decreased in platycodin D-treated cells. The nuclear level of beta-catenin was normally down-regulated during adipogenesis, but platycodin D treatment led to the accumulation of beta-catenin in the nucleus which resulted in the up-regulation of its target genes, cyclin D (CCND) 1 and peroxisome proliferator-activated receptor gamma (PPAR)gamma. The anti-adipogenic effects of platycodin D were significantly attenuated in beta-catenin siRNA-transfected cells compared with those of control siRNA-transfected cells. beta-catenin siRNA transfection significantly recovered the levels of PPARgamma, CCAAT/enhancer binding protein (C/EBP)alpha and fatty acid binding protein (FABP)4 as well as intracellular lipid droplet formation, all of which were reduced by platycodin D treatment. SIGNIFICANCE: WNT/beta-catenin pathway can be used as a therapeutic target of natural compounds for the regulation of adipogenesis.
机译：目的：本研究表明WNT /β-Catenin途径在Platycodon GrandInclorum中发现的天然化合物的抗脂肪蛋白途径中的作用。主要方法：进行使用小干扰RNA（siRNA）转染的基因敲除，以阐明β-连环蛋白在蛋白质抗脂肪蛋白D的关键作用。实时PCR和Western印迹分析MRNA的表达水平和Wnt / beta-catenin途径中的蛋白质。关键发现：在3T3-L1细胞的adipocyte分化期间，通常抑制Wnt /β-连环蛋白途径的成员，而果胶蛋白D显着恢复了Wnt /β-连环蛋白途径。通过Platycodin D治疗增加了稳定β-连环蛋白的令人毛骨悚然的（DVL）2的mRNA和蛋白表达，但胰蛋白酶蛋白质水平诱导β-连环蛋白的降解的蛋白质水平降低。 β-catenin的核水平通常在脂肪发生过程中抑制，但是蛋白酶DO治疗导致核β-连环蛋白的积累导致其靶基因的上调，细胞周期蛋白D（CCND）1和过氧化物增殖剂激活受体γ（PPAR）γ。与对照siRNA转染的细胞的细胞相比，在β-连环蛋白SiRNA转染细胞中显着减弱了蛋白酶蛋白D的抗脂肪效应。 Beta-catenin siRNA转染显着恢复了pparγ，Ccaat /增强子结合蛋白（C / EBP）α和脂肪酸结合蛋白（Fabp）4以及细胞内脂液滴的水平，所有这些都是通过蛋白酶D处理的降低的。意义：WNT /β-连环蛋白途径可用作用于调节脂肪发生的天然化合物的治疗靶标。

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  • 来源
    《Life sciences》 |2011年第12期|共7页
  • 作者

    Lee H; Bae S; Kim YS; Yoon Y;

  • 作者单位

    Department of Microbiology Chung-Ang University College of Medicine Seoul 156-756 Republic of;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 医药、卫生;
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