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首页> 外文期刊>Life sciences >WNT/beta-catenin pathway mediates the anti-adipogenic effect of platycodin D, a natural compound found in Platycodon grandiflorum.
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WNT/beta-catenin pathway mediates the anti-adipogenic effect of platycodin D, a natural compound found in Platycodon grandiflorum.

机译：WNT /β-catenin途径介导桔梗D（一种在桔梗中发现的天然化合物）的抗脂肪形成作用。

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AIMS: This study was conducted to suggest the role of WNT/beta-catenin pathway in the anti-adipogenic effect of platycodin D, a natural compound found in Platycodon grandiflorum. MAIN METHODS: Gene knockdown experiments using small interfering RNA (siRNA) transfection were conducted to elucidate crucial role of beta-catenin in the anti-adipogenic effects of platycodin D. Real-Time PCR and Western blot were used to analyze the expression levels of mRNAs and proteins in the WNT/beta-catenin pathway. KEY FINDINGS: During the adipocyte differentiation of 3 T3-L1 cells, members of the WNT/beta-catenin pathway were normally down-regulated, whereas platycodin D significantly reinstated the WNT/beta-catenin pathway. The mRNA and protein expressions of disheveled (DVL) 2, which stabilize beta-catenin, were increased by platycodin D treatment, but the protein level of AXIN, which induces the degradation of beta-catenin, was decreased in platycodin D-treated cells. The nuclear level of beta-catenin was normally down-regulated during adipogenesis, but platycodin D treatment led to the accumulation of beta-catenin in the nucleus which resulted in the up-regulation of its target genes, cyclin D (CCND) 1 and peroxisome proliferator-activated receptor gamma (PPAR)gamma. The anti-adipogenic effects of platycodin D were significantly attenuated in beta-catenin siRNA-transfected cells compared with those of control siRNA-transfected cells. beta-catenin siRNA transfection significantly recovered the levels of PPARgamma, CCAAT/enhancer binding protein (C/EBP)alpha and fatty acid binding protein (FABP)4 as well as intracellular lipid droplet formation, all of which were reduced by platycodin D treatment. SIGNIFICANCE: WNT/beta-catenin pathway can be used as a therapeutic target of natural compounds for the regulation of adipogenesis.
机译：目的：进行这项研究以表明WNT /β-catenin途径在桔梗D（一种在桔梗中发现的天然化合物）的抗脂肪形成作用中的作用。主要方法：使用小干扰RNA（siRNA）转染进行基因敲低实验，以阐明β-连环蛋白在铂类抗D素的抗脂肪形成作用中的关键作用。采用实时PCR和Western印迹分析mRNA的表达水平和WNT /β-catenin途径中的蛋白质。主要发现：在3个T3-L1细胞的脂肪细胞分化过程中，WNT /β-catenin通路的成员通常被下调，而铂霉素D显着恢复了WNT /β-catenin通路。稳定β-catenin的不整齐（DVL）2的mRNA和蛋白表达通过铂霉素D处理而增加，但是诱导β-catenin降解的AXIN蛋白水平在铂霉素D处理的细胞中降低了。 β-catenin的核水平通常在脂肪形成过程中被下调，但是铂类D处理导致β-catenin在细胞核中积累，从而导致其靶基因cyclin D（CCND）1和过氧化物酶体上调。增殖物激活受体γ（PPAR）γ。与对照siRNA转染的细胞相比，β-cateninsiRNA转染的细胞中platycodin D的抗脂肪形成作用显着减弱。 β-cateninsiRNA转染可显着恢复PPARgamma，CCAAT /增强子结合蛋白（C / EBP）α和脂肪酸结合蛋白（FABP）4的水平，以及细胞内脂质小滴的形成，所有这些都可通过platycodin D处理降低。意义：WNT /β-catenin途径可作为天然化合物的治疗靶标，用于调节脂肪形成。

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