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Effects of N-acetyl-l-cysteine on adhesive strength between breast cancer cell and extracellular matrix proteins after ionizing radiation

机译：N-乙酰-1-半胱氨酸对电离辐射后乳腺癌细胞和细胞外基质蛋白粘合强度的影响

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摘要

Aims: To evaluate the effect of N-acetyl-L-cysteine (LNAC), a common ROS scavenger, on the adhesive affinity between MDA-MB-231 breast cancer cells and extracellular matrix (ECM) proteins after IR. Main methods: Using static cell adhesion assays to determine the effect of various times and duration of LNAC (10 mM) treatment on IR (20 Gy)-altered adhesive affinity between MDA-MB-231 breast cancer cells and ECM, especially fibronectin; using fluorescence dye carboxy- 2,7-dichlorodihydrofluorescein diacetate to determine intracellular levels of ROS; using flow cytometry to determine cell surface integrin β1; and using Western blot analysis to determine vimentin expression. Key findings: Our results indicated that continuously treating the breast cancer cells with LNAC for 24 h, starting immediately after IR, could inhibit IR-induced cell adhesion to ECM proteins at 24 h post-IR. The reduction of cell adhesive affinity was correlated with a down-regulation of IR-induced ROS production and surface expression of activated integrin β1. When the cells were pretreated for 1 h, the inhibitory effects of LNAC were found to be either reduced or completely abrogated followed by 24 h or 2 h treatments, respectively. In addition to cell adhesion, treatment with LNAC inhibited IR-induced expression of vimentin, an epithelial-mesenchymal transition marker (EMT). Significance: The benefits of administering antioxidants during radiation therapy have been the subject of much controversy. Our results suggest that if antioxidant treatment is to be combined with IR therapy, time of administration and treatment duration are important variables to consider.
机译：目的：评价N-乙酰-1-半胱氨酸（LNAC），普通ROS清除剂，在IR后MDA-MB-231乳腺癌细胞和细胞外基质（ECM）蛋白之间的粘合剂亲和力的影响。主要方法：采用静态电池粘附测定测定MDA-MB-231乳腺癌细胞和ECM，特别是纤维蛋白的IR（20μm）altered粘合剂亲和力的不同时间和LNAc（10mm）的持续时间的效果。使用荧光染料羧基-2,7-二氯二硫杂氟呋喃酮霉素，用于确定植物的细胞内水平;使用流式细胞术测定细胞表面整合蛋白β1;并利用Western印迹分析来确定Vimentin表达。关键发现：我们的结果表明，在IR后立即开始使用LNAC的乳腺癌细胞24小时，可以抑制IR后24小时对ECM蛋白的IR诱导的细胞粘附。细胞粘合剂亲和力的减少与IR诱导的ROS产生和活化整合蛋白β1的表面表达的下调相关。当细胞预处理1小时时，发现LNAAc的抑制作用分别减少或完全废除，然后分别为24小时或2小时治疗。除了细胞粘附之外，用LNAC处理抑制了抗石菖蒲的IR诱导的皮，一种上皮 - 间充质转换标志物（EMT）。意义：在放射治疗过程中施用抗氧化剂的益处是有很多争议的主题。我们的研究结果表明，如果抗氧化治疗与IR治疗结合，则管理时间和治疗持续时间是要考虑的重要变量。

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  • 来源
    《Life sciences》 |2013年第21期|共6页
  • 作者

    ChengH.; LeeS.H.; WuS.;

  • 作者单位

    Edison Biotechnology Institute Ohio University Athens OH 45701 United States 118 Konneker;

    Edison Biotechnology Institute Ohio University Athens OH 45701 United States;

    Edison Biotechnology Institute Ohio University Athens OH 45701 United States 118 Konneker;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 医药、卫生;
  • 关键词

    Breast cancer; Cell adhesion; Integrin; Ionizing radiation; LNAC;

    机译：乳腺癌;细胞粘附;整合素;电离辐射;LNAC;

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