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首页> 外文期刊>Life sciences >Effects of N-acetyl-l-cysteine on adhesive strength between breast cancer cell and extracellular matrix proteins after ionizing radiation
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Effects of N-acetyl-l-cysteine on adhesive strength between breast cancer cell and extracellular matrix proteins after ionizing radiation

机译：N-乙酰基-1-半胱氨酸对电离辐射后乳腺癌细胞与细胞外基质蛋白黏附强度的影响

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Aims: To evaluate the effect of N-acetyl-L-cysteine (LNAC), a common ROS scavenger, on the adhesive affinity between MDA-MB-231 breast cancer cells and extracellular matrix (ECM) proteins after IR. Main methods: Using static cell adhesion assays to determine the effect of various times and duration of LNAC (10 mM) treatment on IR (20 Gy)-altered adhesive affinity between MDA-MB-231 breast cancer cells and ECM, especially fibronectin; using fluorescence dye carboxy- 2,7-dichlorodihydrofluorescein diacetate to determine intracellular levels of ROS; using flow cytometry to determine cell surface integrin β1; and using Western blot analysis to determine vimentin expression. Key findings: Our results indicated that continuously treating the breast cancer cells with LNAC for 24 h, starting immediately after IR, could inhibit IR-induced cell adhesion to ECM proteins at 24 h post-IR. The reduction of cell adhesive affinity was correlated with a down-regulation of IR-induced ROS production and surface expression of activated integrin β1. When the cells were pretreated for 1 h, the inhibitory effects of LNAC were found to be either reduced or completely abrogated followed by 24 h or 2 h treatments, respectively. In addition to cell adhesion, treatment with LNAC inhibited IR-induced expression of vimentin, an epithelial-mesenchymal transition marker (EMT). Significance: The benefits of administering antioxidants during radiation therapy have been the subject of much controversy. Our results suggest that if antioxidant treatment is to be combined with IR therapy, time of administration and treatment duration are important variables to consider.
机译：目的：评估IR后常见的ROS清除剂N-乙酰-L-半胱氨酸（LNAC）对MDA-MB-231乳腺癌细胞与细胞外基质（ECM）蛋白之间粘附亲和力的影响。主要方法：使用静态细胞粘附测定法确定LNAC（10 mM）处理的不同时间和持续时间对MDA-MB-231乳腺癌细胞与ECM，特别是纤连蛋白之间IR（20 Gy）改变的粘附亲和力的影响;使用荧光染料羧基-2,7-二氯二氢荧光素二乙酸酯测定细胞内ROS水平;用流式细胞仪测定细胞表面整合素β1;并使用蛋白质印迹分析来确定波形蛋白的表达。关键发现：我们的结果表明，在IR后立即连续用LNAC处理乳腺癌细胞24小时，可以在IR后24 h抑制IR诱导的细胞对ECM蛋白的粘附。细胞粘附亲和力的降低与IR诱导的ROS产生的下调和活化的整联蛋白β1的表面表达相关。当将细胞预处理1 h后，发现LNAC的抑制作用减弱或完全消失，随后分别进行24 h或2 h处理。除细胞粘附外，用LNAC进行的治疗还抑制了IR诱导的波形蛋白表达，波形蛋白是一种上皮-间质转化标记（EMT）。启示：放射治疗期间使用抗氧化剂的好处一直是很多争议的主题。我们的结果表明，如果将抗氧化剂治疗与IR治疗结合使用，则给药时间和治疗持续时间是需要考虑的重要变量。

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