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首页> 外文期刊>RSC Advances >Development of an immunochromatographic lateral flow strip for the simultaneous detection of aminoglycoside residues in milk
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Development of an immunochromatographic lateral flow strip for the simultaneous detection of aminoglycoside residues in milk

机译：用于同时检测牛奶中氨基糖苷残留物的免疫层状横向流动条的研制

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摘要

A colloidal gold-based immunochromatographic strip with a competitive format has been developed for the rapid, simultaneous, semi-quantitative and quantitative detection of several aminoglycoside residues in milk, including gentamicin sulfate (GM), neomycin sulfate (NEO) and kanamycin sulfate (KN). Three monoclonal antibodies against the three corresponding aminoglycosides were conjugated to colloidal gold particles and applied to the conjugate pads of the strip. The competitors [GM-bovine serum albumin (GM-BSA), NEO-BSA and KN-BSA conjugates] of GM, NEO and KN were immobilized onto a nitrocellulose (NC) membrane at three detection zones, T1, T2, and T3, respectively. The minimal cutoff values of the strip were 10 ng mL(-1) for GM, and 100 ng mL(-1) for NEO and KN, which are lower than the maximum residue levels (MRLs) established for aminoglycosides. The IC50 values of the strip were 0.737 ng mL(-1), 8.971 ng mL(-1) and 11.110 ng mL(-1) for GM, NEO and KN respectively. In conclusion, the immunochromatographic lateral flow strip could be used for rapid, simultaneous, semi-quantitative and quantitative detection of GM, NEO and KN residues in milk.
机译：具有竞争形式的胶体金基免疫层析条带来了牛奶中几种氨基糖苷类残留的快速，同时，半定量和定量检测，包括庆大霉素（GM），新霉素硫酸盐（Neo）和卡那霉素硫酸盐（KN ）。将具有三种相应的氨基糖苷的三种单克隆抗体与胶体金颗粒缀合并施加到条带的缀合物垫上。竞争对手[GM-牛血清白蛋白（GM-BSA），GM，NEO和KN的新BSA和KN-BSA缀合物在三个检测区，T1，T2和T3上固定在硝酸纤维素（NC）膜上，分别。条带的最小截止值为GM的10ng（-1），NeO和Kn的100ng ml（-1），其低于为氨基糖苷的最大残留水平（MRL）。条带的IC 50值为GM，Neo和Kn的0.737 ng ml（-1），8.971 ng ml（-1）和11.110ng ml（-1）。总之，免疫色谱横向流动条可用于牛奶中的GM，Neo和Kn残留物的快速，同时，半定量和定量检测。

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  • 来源
    《RSC Advances》 |2018年第17期|共7页
  • 作者

    Sun Yaning; Yang Jifei; Yang Suzhen; Sang Qingbo; Teng Man; Li Qingmei; Deng Ruiguang; Feng Lili; Hu Xiaofei; Zhang Gaiping;

  • 作者单位

    Henan Acad Agr Sci Henan Prov Key Lab Anim Immunol Minist Agr Key Lab Anim Immunol Zhengzhou 450002 Henan Peoples R China;

    Henan Acad Agr Sci Henan Prov Key Lab Anim Immunol Minist Agr Key Lab Anim Immunol Zhengzhou 450002 Henan Peoples R China;

    Henan Acad Agr Sci Henan Prov Key Lab Anim Immunol Minist Agr Key Lab Anim Immunol Zhengzhou 450002 Henan Peoples R China;

    Zhengzhou Ruilong Food Sci &

    Technol Ltd Co Zhengzhou 450064 Henan Peoples R China;

    Henan Acad Agr Sci Henan Prov Key Lab Anim Immunol Minist Agr Key Lab Anim Immunol Zhengzhou 450002 Henan Peoples R China;

    Henan Acad Agr Sci Henan Prov Key Lab Anim Immunol Minist Agr Key Lab Anim Immunol Zhengzhou 450002 Henan Peoples R China;

    Henan Acad Agr Sci Henan Prov Key Lab Anim Immunol Minist Agr Key Lab Anim Immunol Zhengzhou 450002 Henan Peoples R China;

    Henan Acad Agr Sci Inst Agr Econ &

    Informat Zhengzhou 450002 Henan Peoples R China;

    Henan Acad Agr Sci Henan Prov Key Lab Anim Immunol Minist Agr Key Lab Anim Immunol Zhengzhou 450002 Henan Peoples R China;

    Henan Acad Agr Sci Henan Prov Key Lab Anim Immunol Minist Agr Key Lab Anim Immunol Zhengzhou 450002 Henan Peoples R China;

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  • 正文语种 eng
  • 中图分类 化学;
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