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首页> 外文期刊>RSC Advances >Formation of nanopores in DiynePC-DPPC complex lipid bilayers triggered by on-demand photo-polymerization
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Formation of nanopores in DiynePC-DPPC complex lipid bilayers triggered by on-demand photo-polymerization

机译：通过按需光聚合触发的DIYNEPC-DPPC复合脂质双层中纳米孔的形成

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摘要

Vesicles have unique characteristics that enable the release of drugs as well as encapsulation while maintaining biocompatibility. A photo-polymerizable liposome composed of 1,2-bis(10,12-tricosadiynoyl)-sn-glycero-3-phosphocholine (23: 2 DiynePC) has been investigated as vehicles for triggered delivery of drugs to cells. In this study, we confirmed for the first time that supported lipid bilayers (SLBs) prepared with a 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC)/DiynePC mixture generated pores ca. 100-300 nm in size on the membrane after UV polymerization. This direct observation was done by analyzing the SLBs formed with the DPPC/DiynePC mixture by employing atomic force microscopy (AFM) in a liquid environment. However, photo-polymerization did not occur in the 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC)/DiynePC mixed bilayer and pores were not formed. A theoretical study was performed to explore the phase behavior of the lipid mixtures. A coarse-grained model of DiynePC was developed that is comparable with the Martini force field; the parameters were validated against atomistic simulations. Transition from fluidic to gel phase was observed only when DiynePC was mixed with DPPC, whereas the DOPC mixture remained fluidic over the entire domain. This implies a correlation between the formation of DiynePC-rich gel phase domains and the generation of pores after polymerization. The size of the pores were found to be controlled by the amount of polymerizable lipid which results in higher release rate of encapsulated calcein from the vesicles with larger pores.
机译：囊泡具有独特的特性，使药物释放以及封装在保持生物相容性的同时。已经研究了由1,2-BIS（10,12-三胞嘧啶酰亚炔酰基）-SN-甘油-3-普膦酰胺（23：2 DIYnepc）组成的光聚合脂质体已被研究作为用于将药物递送给细胞的车辆。在这项研究中，我们首次证实了用1,2-二普咪酰-N-甘油-3-普华啉（DPPC）/ DIYNEPEPC混合物制备的脂质双层（SLB）的第一次得到了脂质双层（SLB）。紫外线聚合后膜上的100-300nm尺寸。通过在液体环境中使用原子力显微镜（AFM）通过分析用DPPC / DIYNEPEPC混合物形成的SLB来完成这种直接观察。然而，在1,2-Dioleyl-sn-甘油-3-普酞啉（DOPC）/ Diynepc混合双层和孔未形成光聚合。进行理论研究以探讨脂质混合物的相行为。开发了一种与马提尼强力场相当的DIYNEPC粗粒模型;参数验证了反对原子模拟。只有当DOYNEPC与DPPC混合时，才观察到从流体到凝胶相的转变，而DOPC混合物在整个结构域上仍然流体。这意味着在聚合后富含富合作的凝胶相位域的形成与孔的产生之间的相关性。发现孔的尺寸由可聚合脂质的量控制，这导致从具有较大孔的囊泡的包封钙蛋白的较高释放速率。

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  • 来源
    《RSC Advances》 |2018年第49期|共7页
  • 作者

    Chun Min Jung; Choi Yeol Kyo; Ahn Dong June;

  • 作者单位

    Korea Univ Dept Chem &

    Biol Engn Seoul 02841 South Korea;

    Korea Univ Dept Chem &

    Biol Engn Seoul 02841 South Korea;

    Korea Univ Dept Chem &

    Biol Engn Seoul 02841 South Korea;

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  • 正文语种 eng
  • 中图分类 化学;
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