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首页> 外文期刊>RSC Advances >Magnetic-propelled Fe3O4-chitosan carriers enhance l-asparaginase catalytic activity: a promising strategy for enzyme immobilization
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Magnetic-propelled Fe3O4-chitosan carriers enhance l-asparaginase catalytic activity: a promising strategy for enzyme immobilization

机译：磁力推进的Fe3O4-壳聚糖载体增强L-天冬酰胺酶催化活性：酶固定化的有希望的策略

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摘要

Magnetic-propelled carriers comprising magnetic Fe3O4-chitosan nanoparticles were immobilized with l-asparaginase (l-ASNase). The enzyme displayed enhanced catalytic activity in a weak magnetic field, and thermal and pH stabilities. The conjugated l-ASNase presented higher thermostability and wider range of pH stability in comparison with those of free l-ASNase. Moreover, the reusability of conjugated l-ASNase significantly improved after immobilization and it retained 60.5% of its initial activity after undergoing 16 cycles. The conjugated l-ASNase maintained more than 50% and 48% initial activity after 4 weeks of storage at 4 degrees C and room temperature, respectively. Furthermore, we reveal that the activity of conjugated l-ASNase onto magnetic Fe3O4-chitosan particles increased by about 3-fold in the weak magnetic field at certain frequencies and flux density compared with that of free l-ASNase. Considering these excellent attributes, the magnetic-propelled mechanism in the transporting and activation of l-ASNase can be used by enhancing the catalytic activity, stability, and efficiency in vital implications for medicinal biotechnology.
机译：将包含磁性Fe3O4-壳聚糖纳米颗粒的磁力推进载体用L-天冬酰胺酶（L- asnase）固定。酶在弱磁场中显示出增强的催化活性，以及​​热和pH稳定性。与游离L-Asnase的那些相比，缀合的L-Asnase呈现更高的热稳定性和更广泛的pH稳定性。此外，固定后共轭L-Asnase的可重用性显着改善，并且在进行16个循环后，它在其初始活性中保留了60.5％。在4℃和室温下储存4周后，共轭L-Asnase在4周后保持超过50％和48％的初始活性。此外，我们揭示了与游离L-Asnase的磁磁场中的磁Fe3O4-壳聚糖颗粒上的缀合的L- asnase的活性在弱磁场中增加了约3倍。考虑到这些优异的属性，通过提高药物生物技术至关重要的催化活性，稳定性和效率，可以使用转运和激活L- asnase的磁力推进机制。

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  • 来源
    《RSC Advances》 |2018年第63期|共13页
  • 作者

    Ates Burhan; Ulu Ahmet; Koytepe Suleyman; Ali Noma Samir Abbas; Kolat Veli Serkan; Izgi Tekin;

  • 作者单位

    Inonu Univ Fac Sci &

    Arts Dept Chem TR-44280 Malatya Turkey;

    Inonu Univ Fac Sci &

    Arts Dept Chem TR-44280 Malatya Turkey;

    Inonu Univ Fac Sci &

    Arts Dept Chem TR-44280 Malatya Turkey;

    Inonu Univ Fac Sci &

    Arts Dept Chem TR-44280 Malatya Turkey;

    Inonu Univ Fac Sci &

    Arts Dept Phys TR-44280 Malatya Turkey;

    Inonu Univ Fac Sci &

    Arts Dept Phys TR-44280 Malatya Turkey;

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  • 正文语种 eng
  • 中图分类 化学;
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