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首页> 外文期刊>RSC Advances >A disposable microcapsule array chip fabricated by ice printing combined with isothermal amplification for Salmonella DNA detection
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A disposable microcapsule array chip fabricated by ice printing combined with isothermal amplification for Salmonella DNA detection

机译：由冰印刷制造的一次性微胶囊阵列芯片与沙门氏菌DNA检测的等温扩增相结合

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摘要

In this work, a novel microcapsule array chip was fabricated for the detection of Salmonella DNA by integrating an ice-printing technique with DNA isothermal amplification. Reaction solutions were previously sealed in the microcapsule array chip via ice printing. To protect the relatively fragile DNA isothermal amplification system, an extra polystyrene (PS) film was introduced to isolate the reaction solution from photopolymer precursor, which was proved to be a vital step for providing a clean and stable environment for DNA amplification reaction. Detection operation can be done by simply injecting sample DNA into the microcapsule by an easily accessible syringe, and the result can be directly obtained through color change within 90 minutes. This method shows good sensitivity, specificity and stability.
机译：在这项工作中，通过将冰印刷技术与DNA等温扩增的冰印刷技术集成来制造一种新的微胶囊阵列芯片，用于检测沙门氏菌DNA。 预先通过冰印刷将反应溶液在微胶囊阵列芯片中密封。 为了保护相对脆弱的DNA等温扩增系统，引入额外的聚苯乙烯（PS）膜以将反应溶液从光聚合物前体分离，这被证明是提供用于DNA扩增反应的清洁和稳定环境的重要步骤。 通过易于可触及的注射器将样品DNA喷射到微胶囊中，可以通过将样品DNA注入微胶囊来完成检测操作，并且可以通过90分钟内通过颜色变化直接获得结果。 该方法显示出良好的敏感性，特异性和稳定性。

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  • 来源
    《RSC Advances》 |2018年第69期|共6页
  • 作者

    He Enqi; Cao Ting; Cai Liangyuan; Guo Dan; Zhou Yinglin; Zhang Xinxiang; Li Zhihong;

  • 作者单位

    Tsinghua Univ State Key Lab Tribol Dept Mech Engn Beijing 100084 Peoples R China;

    Peking Univ Coll Chem Key Lab Bioorgan Chem &

    Mol Engn BNLMS Minist Educ Beijing 100871 Peoples R China;

    Peking Univ Coll Chem Key Lab Bioorgan Chem &

    Mol Engn BNLMS Minist Educ Beijing 100871 Peoples R China;

    Tsinghua Univ State Key Lab Tribol Dept Mech Engn Beijing 100084 Peoples R China;

    Peking Univ Coll Chem Key Lab Bioorgan Chem &

    Mol Engn BNLMS Minist Educ Beijing 100871 Peoples R China;

    Peking Univ Coll Chem Key Lab Bioorgan Chem &

    Mol Engn BNLMS Minist Educ Beijing 100871 Peoples R China;

    Peking Univ Inst Microelect Natl Key Lab Sci &

    Technol Micro Nano Fabricat Beijing 100871 Peoples R China;

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  • 正文语种 eng
  • 中图分类 化学;
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