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Hematite iron oxide nanoparticles: apoptosis of myoblast cancer cells and their arithmetical assessment

机译：赤铁矿氧化铁纳米粒子：肌细胞癌细胞的凋亡及其算术评估

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摘要

Hematite (-Fe2O3) forms iron oxide nanoparticles (NPs) which are thermally stable and have various electrochemical and optochemical applications. Due to their wide applicability, the present work was designed to form the hematite phase of iron oxide (Fe(2)O(3)NPs) NPs prepared via a solution process. Their cytological performance was checked with C2C12 cells. The crystalline property of the NPs was examined with X-ray diffraction patterns (XRD) and it was found that the size of the particles formed ranged from 12 to 15 nm. Structural information was also identified via field emission scanning electron microscopy (FESEM) and transmission electron microscopy (TEM), which again confirmed that the size of each NP is about 12-15 nm. Surface topographical analysis was done via atomic force microscopy (AFM), which reveals that the size of the distance between two particles is in the range of 12 +/- 3 nm. The C2C12 cells were cultured in a humidified environment with 5% CO2 and were checked via a microscope. The Fe(2)O(3)NPs were used for cytotoxic evaluation against C2C12 cells. A MTT (3-(4,5-dimethyl thiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay was utilized to check the viability of cells in a dose-dependent (100 ng mL(-1), 500 ng mL(-1) or 1000 ng mL(-1)) manner. The morphology of the cells under the influence of Fe(2)O(3)NPs for live and dead cells in a wet environment was confirmed via confocal laser scanning microscopy (CLSM). The apoptosis caused due to the Fe(2)O(3)NPs was evaluated in presence of caspases 3/7 with GAPDH genes, which confirmed the upregulation that is responsible in caspase 3/7 genes, with treatment of C2C12 at low (500 ng mL(-1)) and high (1000 ng mL(-1)) doses of Fe(2)O(3)NPs. Analytical studies were also performed to authenticate the obtained data for Fe(2)O(3)NPs using parameters such as precision, accuracy, linearity, limits of detection (LOD) and limit of quantitation (LOQ), quantitative recoveries and relative standard deviation (RSD). The analyses play a significant role in investigating the large effect of Fe(2)O(3)NPs on C2C12 cells.
机译：赤铁矿（-Fe2O3）形式的氧化铁纳米颗粒（NP），其是热稳定的，并且具有各种电化学和光化学的应用程序。由于其广泛的适用性，本工作的目的是要形成氧化铁的赤铁矿相经由溶液方法制备的铁（Fe（2）O（3）的NP）的NP。他们的细胞学表现与C2C12细胞检查。所述纳米颗粒的结晶性与X射线衍射图（XRD）进行了检查，发现该颗粒的尺寸形成的范围从12至15纳米。结构信息经由场发射扫描还确定电子显微镜（FESEM）和透射电子显微镜（TEM），这再次确认了每个NP的尺寸为约12-15纳米。表面形貌分析通过原子力显微镜（AFM），其揭示了距离的两个粒子间的尺寸在12 +/- 3 nm范围内完成的。在C2C12细胞在具有5％CO 2的潮湿环境中培养，并通过显微镜检查。铁（2）O（3）的NP被用于对C2C12细胞的细胞毒性的评价。甲MTT（3-（4,5-二甲基噻唑-2-基）-2,5-二苯基溴化四）测定用于检查细胞的生存力在剂量依赖的（100毫微克毫升（-1），500纳克毫升（-1）或1000纳克毫升（-1））的方式。细胞的Fe的影响下的形态（2）O（3）的NP用于在潮湿环境中活的和死的细胞通过共聚焦激光扫描显微镜（CLSM）得到了证实。 （2）O（3）的NP与GAPDH基因，这证实了上调，其负责在胱天蛋白酶3/7的基因，与治疗C2C12在低温胱天蛋白酶3/7的存在下评价（由于引起Fe的凋亡500纳克毫升（-1））和高（1000纳克毫升（-1））剂量的Fe的（2）O（3）的NP。还进行分析研究，以用于铁验证所获得的数据（2）O（3）的NP使用的参数，如精度，准确度，线性，检测（LOD）和定量限（LOQ）的限制的限制，定量回收率和相对标准偏差（RSD）。该分析调查的Fe（2）O（3）纳米粒子对C2C12细胞的作用大发挥显著作用。

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  • 来源
    《RSC Advances》 |2018年第44期|共10页
  • 作者

    Wahab Rizwan; Khan Farheen; Al-Khedhairy Abdulaziz A.;

  • 作者单位

    King Saud Univ Coll Sci Zool Dept POB 2455 Riyadh 11451 Saudi Arabia;

    Taibah Univ Fac Sci Chem Dept Yanbu Saudi Arabia;

    King Saud Univ Coll Sci Zool Dept POB 2455 Riyadh 11451 Saudi Arabia;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 化学;
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