• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>RSC Advances >A sensitive fluorescent probe for alkaline phosphatase and an activity assay based on the aggregation-induced emission effect
【24h】

A sensitive fluorescent probe for alkaline phosphatase and an activity assay based on the aggregation-induced emission effect

机译：基于聚集诱导的发射效应的碱性磷酸酶和活性测定的敏感荧光探针

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

In this work, we report a fluorescent probe (TPEQN-P) for detecting alkaline phosphatase (ALP) with high sensitivity and monitoring its activity based on the specific aggregation-induced emission (AIE) effect. TPEQN-P can be constructed by conjugating the phosphate moiety into an AIE molecule (TPE-QI) through a reactive p-hydroxybenzyl group, which exhibits very weak emission in aqueous media due to its good water solubility. In the presence of ALP, TPEQN-P undergoes dephosphorylation and releases the hydrolysis product TPE-QI, which is intensely fluorescent because of its poor water solubility. The detection limit for ALP using TPEQN-P can be as low as 0.0077 U L-1 with a linear range of 0-30 mU mL(-1) in solution. TPEQN-P also shows excellent applicability in serum samples, demonstrating potential applications in clinical diagnosis and biomedical research. TPEQN-P also can be applied for the determination of ALP activity and in ALP inhibitor screening.
机译：在这项工作中，我们报告了一种荧光探针（TPEQN-P），用于检测具有高灵敏度的碱性磷酸酶（ALP）并基于特异性聚集诱导的发射（AIE）效应来监测其活性。 TPEQN-P可以通过将磷酸盐部分通过反应性对羟基苄基缀合成A形分子（TPE-QI）来构建，其由于其良好的水溶性而在水性介质中发出非常弱。 在ALP存在下，TPEQN-P经历去磷酸化并释放水解产物TPE-QI，这是强烈的荧光溶解度。 使用TPEQN-P的ALP的检测限度可以低至0.0077u L-1，在溶液中具有0-30μm1（-1）的线性范围。 TPEQN-P还显示出在血清样品中的优异适用性，展示了临床诊断和生物医学研究中的潜在应用。 TPEQN-P也可用于测定ALP活性和ALP抑制剂筛选。

著录项

  • 来源
    《RSC Advances》 |2018年第27期|共6页
  • 作者

    Zhang Wenjuan; Yang Hanxiao; Li Nan; Zhao Na;

  • 作者单位

    Shaanxi Normal Univ Key Lab Macromol Sci Shaanxi Prov Key Lab Appl Surface &

    Colloid Chem Minist Educ Sch Chem &

    Chem Engn Xian 710119 Shaanxi Peoples R China;

    Shaanxi Normal Univ Key Lab Macromol Sci Shaanxi Prov Key Lab Appl Surface &

    Colloid Chem Minist Educ Sch Chem &

    Chem Engn Xian 710119 Shaanxi Peoples R China;

    Shaanxi Normal Univ Key Lab Macromol Sci Shaanxi Prov Key Lab Appl Surface &

    Colloid Chem Minist Educ Sch Chem &

    Chem Engn Xian 710119 Shaanxi Peoples R China;

    Shaanxi Normal Univ Key Lab Macromol Sci Shaanxi Prov Key Lab Appl Surface &

    Colloid Chem Minist Educ Sch Chem &

    Chem Engn Xian 710119 Shaanxi Peoples R China;

  • 收录信息
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 化学;
  • 关键词

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号