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首页> 外文期刊>RSC Advances >Ultrasensitive enzyme-free fluorescent detection of VEGF(165) based on target-triggered hybridization chain reaction amplification
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Ultrasensitive enzyme-free fluorescent detection of VEGF(165) based on target-triggered hybridization chain reaction amplification

机译：基于目标触发的杂交链反应扩增的VEGF（165）的超敏感酶无荧光检测

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摘要

Sensitive detection of vascular endothelial growth factor (VEGF(165)) is important for early cancer disease diagnosis in the clinic. A sensitive fluorescent sensing platform for VEGF(165) detection is developed in this work. It is based on a target-triggered hybridization chain reaction (HCR) and graphene oxide (GO) selective fluorescence quenching. In this assay, in the presence of the VEGF(165), the hairpin structure of Hp opens up and the initiation sequence will be exposed to Hp1 to open its hairpin structure. Then the opened Hp1 hybridizes with Hp2 to expose the complementary sequence of Hp1 which hybridizes with Hp1 again by HCR. Thus HCR would be initiated, generating super-long dsDNA. After the HCR, the double strands of the HCR product cannot be adsorbed on the GO surface. As a result, the HCR product gives a strong fluorescence signal which is dependent on the concentration of VEGF(165). By using VEGF(165) as a model analyte, the assay provides a highly sensitive fluorescence detection method for VEGF(165) with a detection limit down to 20 pg mL(-1). The proposed aptasensing strategy based on target-triggered HCR amplification can thus be realized. It was successfully applied to the determination of VEGF(165) in spiked human serum, urine and saliva. Therefore, it can easily have wide applications in the diagnosis of vital diseases.
机译：血管内皮生长因子的敏感性检测（VEGF（165））对于临床癌症疾病诊断至关重要。在这项工作中开发了一种用于VEGF（165）检测的敏感荧光传感平台。它基于目标触发的杂交链反应（HCR）和石墨烯氧化物（GO）选择性荧光猝灭。在该测定中，在VEGF（165）存在下，HP的发夹结构打开并将起始序列暴露于HP1以打开其发夹结构。然后，已打开的HP1与HP2杂交，暴露HP1的互补序列，其再次通过HP1与HP1杂交。因此，将启动HCR，产生超长的DSDNA。在HCR之后，HCR产物的双链不能吸附在去表面上。结果，HCR产物具有强荧光信号，其取决于VEGF（165）的浓度。通过使用VEGF（165）作为模型分析物，测定法为VEGF（165）提供高敏感的荧光检测方法，其中检测限达20pg ml（-1）。因此，可以实现基于目标触发的HCR扩增的提出的Aptasencing策略。它成功地应用于尖刺人血清，尿液和唾液中的VEGF（165）的测定。因此，它可以很容易地具有广泛的应用在诊断生命疾病中。

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  • 来源
    《RSC Advances》 |2018年第46期|共6页
  • 作者

    Zhou Qingzhen; Yan Hongxia; Ran Fengying; Cao Jianjun; Chen Long; Shang Bing; Chen Hao; Wei Jian; Chen Qinhua;

  • 作者单位

    Hubei Univ Med Affiliated Dongfeng Hosp Shiyan 442008 Hubei Peoples R China;

    Hubei Canc Hosp Dept Radiotherapy 116 South Zuodaoquan Rd Wuhan 430074 Hubei Peoples R China;

    Hubei Univ Med Affiliated Dongfeng Hosp Shiyan 442008 Hubei Peoples R China;

    Hubei Univ Med Affiliated Dongfeng Hosp Shiyan 442008 Hubei Peoples R China;

    Hubei Univ Med Affiliated Dongfeng Hosp Shiyan 442008 Hubei Peoples R China;

    Hubei Univ Med Affiliated Dongfeng Hosp Shiyan 442008 Hubei Peoples R China;

    Hubei Univ Med Affiliated Dongfeng Hosp Shiyan 442008 Hubei Peoples R China;

    Hubei Univ Med Affiliated Dongfeng Hosp Shiyan 442008 Hubei Peoples R China;

    Hubei Univ Med Affiliated Dongfeng Hosp Shiyan 442008 Hubei Peoples R China;

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  • 正文语种 eng
  • 中图分类 化学;
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