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首页> 外文期刊>RSC Advances >Dendritic cells trigger imbalance of Th1/Th2 cells in silica dust exposure rat model via MHC-II, CD80, CD86 and IL-12
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Dendritic cells trigger imbalance of Th1/Th2 cells in silica dust exposure rat model via MHC-II, CD80, CD86 and IL-12

机译：树突状细胞通过MHC-II，CD80，CD86和IL-12触发二氧化硅粉尘大鼠模型中Th1 / Th2细胞的不平衡

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摘要

Silicosis is one of the most common occupational respiratory diseases caused by inhaling silica dust over a prolonged period of time, and the progression of silicosis is accompanied with chronic inflammation and progressive pulmonary fibrosis, in which dendritic cells (DCs), the most powerful antigen presentation cell (APC) in the immune response, play a crucial role. To investigate the role of DCs in the development of silicosis, we established an experimental silicosis rat model and examined the number of DCs and alveolar macrophages (AMs) in lung tissues using immunofluorescence over 84 days. Additionally, to obtain an overview of the immunological changes in rat lung tissues, a series of indicators including Th1/Th2 cells, IFN-gamma, IL-4, MHC-II, CD80/86 and IL-12 were detected using flow cytometry and an enzyme-linked immunosorbent assay (ELISA) as well as a real-time polymerase chain reaction (PCR) assay. We observed that the number of DCs slightly increased at the inflammatory stage, and it increased significantly at the final stage of fibrosis. Polarization of Th1 cells and IFN-g expressions were dominant during the inflammatory stage, whereas polarization of Th2 cells and IL-4 expressions were dominant during the fibrotic stage. The subsequent mechanistic study found that the expressions of MHC-II, CD80/86 and IL-12, which are the key molecules that connect DCs and Th cells, changed dynamically in the experimental silicosis rat model. The data obtained in this study indicated that the increase in DCs may contribute to polarization of Th1/Th2 cells via MHC-II, CD80/86, and IL-12 in silica dust-exposed rats.
机译：矽肺是在长时间吸入二氧化硅灰尘引起的最常见的职业呼吸系统疾病之一，矽肺的进展伴有慢性炎症和渐进性肺纤维化，其中树突细胞（DCS），最强大的抗原呈现细胞（APC）在免疫应答中，起到至关重要的作用。为了探讨DCS在矽肺病发展中的作用，我们建立了一种实验性矽肺大鼠模型，并在84天内使用免疫荧光检查肺组织中的DC和肺泡巨噬细胞（AMS）的数量。另外，通过流式细胞术检测，为了获得大鼠肺组织中的免疫变化，包括Th1 / Th2细胞，IFN-γ，IL-4，MHC-II，CD80 / 86和IL-12的一系列指示剂酶联免疫吸附测定（ELISA）以及实时聚合酶链反应（PCR）测定。我们观察到炎症期的DC数量略微增加，并且在纤维化的最后阶段显着增加。在炎症期期间，Th1细胞和IFN-G表达的偏振是显性的，而TH2细胞和IL-4表达的偏振在纤维化阶段期间显着。随后的机械研究发现，MHC-II，CD80 / 86和IL-12的表达，其是连接DC和TH细胞的关键分子，在实验性矽肺大鼠模型中动态地改变。本研究中获得的数据表明，DC的增加可能通过MHC-II，CD80 / 86和IL-12在二氧化硅暴露大鼠中有助于Th1 / Th2细胞的偏振。

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  • 来源
    《RSC Advances》 |2018年第46期|共8页
  • 作者

    Bao Lei; Hao Changfu; Liu Suna; Zhang Lin; Wang Juan; Wang Di; Li Yiping; Yao Wu;

  • 作者单位

    Zhengzhou Univ Sch Publ Hlth 100 Sci Ave Zhengzhou 450001 Henan Peoples R China;

    Zhengzhou Univ Sch Publ Hlth 100 Sci Ave Zhengzhou 450001 Henan Peoples R China;

    Zhengzhou Univ Affiliated Hosp 3 Zhengzhou 450001 Henan Peoples R China;

    Zhengzhou Univ Sch Publ Hlth 100 Sci Ave Zhengzhou 450001 Henan Peoples R China;

    Hebei Gen Hosp Shijiazhuang 050000 Hebei Peoples R China;

    Zhengzhou Univ Sch Publ Hlth 100 Sci Ave Zhengzhou 450001 Henan Peoples R China;

    Zhengzhou Univ Sch Publ Hlth 100 Sci Ave Zhengzhou 450001 Henan Peoples R China;

    Zhengzhou Univ Sch Publ Hlth 100 Sci Ave Zhengzhou 450001 Henan Peoples R China;

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  • 正文语种 eng
  • 中图分类 化学;
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