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首页> 外文期刊>RSC Advances >Cadmium specific proteomic responses of a highly resistant Pseudomonas aeruginosa san ai
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Cadmium specific proteomic responses of a highly resistant Pseudomonas aeruginosa san ai

机译：镉特异性蛋白质组学反应的高抗性假单胞菌铜绿假单胞菌San Ai

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Pseudomonas aeruginosa san ai is a promising candidate for bioremediation of cadmium pollution, as it resists a high concentration of up to 7.2 mM of cadmium. Leaving biomass of P. aeruginosa san ai exposed to cadmium has a large biosorption potential, implying its capacity to extract heavy metal from contaminated medium. In the present study, we investigated tolerance and accumulation of cadmium on protein level by shotgun proteomics approach based on liquid chromatography and tandem mass spectrometry coupled with bioinformatics to identify proteins. Size exclusion chromatography was used for protein prefractionation to preserve native forms of metalloproteins and protein complexes. Using this approach a total of 60 proteins were observed as up-regulated in cadmium-amended culture. Almost a third of the total numbers of up-regulated were metalloproteins. Particularly interesting are denitrification proteins which are over expressed but not active, suggesting their protective role in conditions of heavy metal exposure. P. aeruginosa san ai developed a complex mechanism to adapt to cadmium, based on: extracellular biosorption, bioaccumulation, the formation of biofilm, controlled siderophore production, enhanced respiration and modified protein profile. An increased abundance of proteins involved in: cell energy metabolism, including denitrification proteins; amino acid metabolism; cell motility and posttranslational modifications, primarily based on thiol-disulfide exchange, were observed. Enhanced oxygen consumption of biomass in cadmium-amended culture versus control was found. Our results signify that P. aeruginosa san ai is naturally well equipped to overcome and survive high doses of cadmium and, as such, has a great potential for application in bioremediation of cadmium polluted sites.
机译：铜绿假单胞菌San Ai是镉污染生物修复的有希望的候选者，因为它抵抗高达7.2毫米的镉。将铜绿假单胞菌的生物量留给镉暴露于镉的生物吸附电位大，暗示其从污染介质中提取重金属的能力。在本研究中，我们通过基于液相色谱法和串联质谱法与生物信息学相结合以鉴定蛋白质的霰弹枪蛋白质组学方法研究镉对蛋白质水平对蛋白质水平的耐受性和积累。尺寸排阻色谱法用于蛋白质预选，以保留原生形式的金属蛋白和蛋白质复合物。使用这种方法，在镉修正的培养物中观察到总共60个蛋白质。上调总数的几乎三分之一是金属蛋白。特别有趣的是反硝化蛋白，其被表达但不活跃，表明它们在重金属暴露条件下的保护作用。 P. Aerginosa San Ai开发了一种复杂的机制，适应镉，基于：细胞外生物沉积，生物累积，生物膜的形成，控制的呼吸和改性蛋白质概况。增加的蛋白质含量增加：细胞能量代谢，包括反硝化蛋白;氨基酸代谢;观察到细胞运动和后期改性，主要是基于硫醇二硫化物交换。发现了增强镉修正培养的生物质的氧气消耗与对照。我们的结果表明，P. Aeruginosa San Ai自然能够康复和存活高剂量的镉，因此在镉污染部位的生物修复中具有巨大潜力。

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